AOAC SPIFAN ERP Meeting Book-March 16, 2016

FOS-01 w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Report: SPIFAN FOS-01 Single Lab Validation (SLV) for fructan in Infant Formula and Adult Nutritionals.

1. Introduction. During the September session (2014) at Boca Raton, the SPIFAN GOS/FOS working group approved the AOAC 997.08 method for further evaluation for a SLV for the FOS determination. The AOAC 997.08 method is an enzymatic, chromatographic method for the determination of fructans in food and feed products. At the time of approval of the AOAC method (1997) a collaborative study was performed on samples with different matrices. This method is applied on a daily basis in our lab. The method gives freedom to apply a chromatographic technique as long as the determination of the sugars can be done accurate and without interference. Depending on the matrix of the sample, we decide which chromatographic technique is performed, being GC, HPLC or HPAEC. For dairy samples our preferred method is GC because of the complexity of the matrix. A yearly proficiency test is organized by DLA in Germany where different labs are asked to analyze for the fructan content of a double blind commercial sample. More info can be found on http://www.dla-lvu.de. The matrix changes every year. Our lab participates from 2008 on. A Test Materials Kit, ordered at AOAC, and Covance provided different products of infant and adult nutritionals like listed in the table below. As a first step the 3 placebo samples and the 12 fortified samples were screened in duplicate for the presence of fructans. 3 fortified samples (J4G-00019, J4G-00974 and J4G-00980) and also one of the placebo samples J4G-00013 were found to contain fructans. The pure products Orafti HP(inulin), Orafti P95(FOS) and Nutraflora P95(FOS) were screened in duplicate by 3 different chromatographic techniques, being GC, HPAEC and HPLC. Influence of the applied chromatographic technique is negligible as all results for the fructan content are on the same level. The setup of the recovery (spiking) scheme was done in such a way that the 3 different raw products were used over the whole range. 2. Methodology 2.1Samples for SLV.

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