AOAC SPIFAN ERP Meeting Book-March 16, 2016

FOS-01 w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Nutraflora P-95 11101 (FOS powder)

Raw materials

Sample nr

J4G-00010

GC

HPAEC

HPLC

1

2

1

2

1

2

Fructan g/100g sample

90,8 96,1 94,5

91,4 96,2 95,1

90,7 96,1 94,4

90,9 96,2 94,5

90,6 96,1 94,3

90,9 96,2 94,5

DM

%

Fructan

g/100g DM

Orafti P95 (FOS)

Raw materials

Sample nr

J4G-00011

GC

HPAEC

HPLC

1

2

1

2

1

2

Fructan g/100g sample

90,5 96,8 93,5

91,0 96,8 94,0

92,5 96,8 95,6

93,3 96,8 96,4

90,7 96,8 93,7

90,6 96,8 93,6

DM

%

Fructan

g/100g DM

Orafti HP (inulin)

Raw materials

Sample nr

J4G-00012

GC

HPLC

1

2

1

2

Fructan g/100g sample

96,5 96,8 99,7

96,3 96,8 99,4

96,2 96,8 99,3

96,2 96,8 99,4

DM

%

Fructan

g/100g DM

The scheme of spiking/recovery was set up in such a way that all of the 3 different raw materials were used in different concentrations. To have included the different matrices of the infant and adult powders, each of the matrices with amounts of fructans under the LOQ were spiked.

Raw material

spike concentration g/100g reconstituted product

Nutraflora

P95

HP

0,05

1

5 x

x

SRM 1849a

CLC10-b

infant powder partially hydr Milk based infant powder partially hydr Soy based Adult powder low fat Infant elemental powder Infant formula milk based Infant formula powder Soy based Infant formula RTF Milk based

x

x

1172572116

x

x

117257651Z 00394RF00 00403RF00 D04HTCVV

x

x

x

x

x

x x

x

E29JVLV

SPIFAN Control milk form EV4H2R

x

x

x

x

Adult nutr RTF high Protein

00730RF00

p.5