AOAC SPIFAN ERP Meeting Book-March 16, 2016

FOS-01 w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Each spiked sample was analyzed in duplicate on 3 different days and handled by different operators. 3. Results 3.1 Precision There were found 4 products to contain fructans. Each sample was analyzed in double on 6 different days so 12 analyses in total per sample.

SPIFAN Code

ID number

Conc. Level

Product

RSDr

RSD iR

J4G- 00019 J4G- 00980 J4G- 00013 J4G- 00974

Adult nutr powder Milk Protein based

11750017V3

0,59

3,2

3,7

Adult nutr RTF high Fat

00729RF00

0,06

8,0

9,7

Child formula powder

00411RF00

0,35

4,5

4,8

Child formula powder

00412RF00

0,28

9,5

10,1

The analytical range as proposed at the AOAC SMPR 2014.002 was 0.03 to 5.0 g/100g reconstituted final product. The concentrations obtained are not really covering this proposed range. The lower the concentration the higher the RDSr as the error of the method increases. At the time of the collaborative study of AOAC 997.08 the RSDr ranged from 2.9 to 5.8%, but concentrations of fructan were higher, 4.6 to 42 g/100g sample. 3.2 Accuracy 9 different matrices were spiked at different levels covering the proposed range from 0.05 to 5 g/100g reconstituted product. The 3 raw materials available were used for this spiking.

p.6