AOAC SPIFAN ERP Meeting Book-March 16, 2016

FOS-01 w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

4.

Calculation:

For each standard solution, the response factor of each compound is calculated as follows:

R = A x M° M x A°

R

=

response factor of the compound weight of the compound in mg/50 cm 3

M = A = M° = A° =

peak area of the compound weight of IS in mg/50 cm 3

peak area IS

For each compound a calibration curve of the type, y=ax, will be obtained. With this curve all the concentrations of the compounds present in the sample can be calculated. (r2= min 0.995) Formula:

C = M° x A x 100 a A° W

C = concentration of the compound in mg/100 g sample A = peak area of the compound M° = weight of IS in mg/50 cm 3 A° = peak area IS W = weight of sample in g/50 cm 3

The reintegration and calculations are performed with the software ATLAS or similar.

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