AOAC SPIFAN ERP Meeting Book-March 16, 2016

FOS-01 MEF Form FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 1

Accuracy ‐ Spike Recovery

Spike Recovery

Nr. replicates  (n)

Product

ID number

Analyte

 + Spike Level 1

SPIFAN CODE

Native level

Avg (%)

RSD %

SRM 1849a

CLC10‐b

J4G‐00018

fructan

6

<0,03

98.2

1.7 spiked with Orafti P95

infant powder partially hydr Milk based 1172572116

J4G‐00020

fructan

6

<0,03

99.8

0.4 spiked with Nutraflora P95

infant powder partially hydr Soy based 117257651Z

J4G‐00971 J4G‐00973 J4G‐00975 J4G‐00976 J4G‐00977

fructan fructan fructan fructan fructan

6 6 6 6 6

<0,03 <0,03 <0,03 <0,03 <0,03

101.4 107.1 101.5

1.7 spiked with HP inulin

Adult nutr  powder low fat Infant elemental powder

00394RF00 00403RF00 D04HTCVV

7.2 spiked with Nutraflora P95 3.5 spiked with Orafti P95

Infant formula powder Milk based Infant formula powder Soy based

96.9 98.4

1.6 spiked with HP inulin

E29JVLV

5.5 spiked with Nutraflora P95

SPIFAN Control milk  form EV4H2R

Infant formula RTF Milk based

J4G‐00978

fructan

6

<0,03

100.8

2.3

spiked with Orafti P95

Adult nutr RTF high Protein

00730RF00

J4G‐00979

fructan

6

<0,03

98.8

1.2 spiked with HP inulin

Global Mean % 100.3

2.8

#DIV/0!

#DIV/0!

Additional information to be completed by SD

Were all spike recovery samples prepared on multiple days? Were spike recoveries calculated from theoretical additions? Or was spiking solution analyzed and the results used to calculate spike recoveries? Was a portion of the product spiked? Were all spike recovery samples taken from the same spiked product? Were multiple portions of the product spiked and each spiked product analyzed? Were products weighed into extraction vessels before spiking? Were products spiked before they were weighed into extraction vessels? Were all spike recovery samples prepared on the same day?

yes no yes yes

no no

yes yes no

Nutraflora P95 (FOS)/Orafti P95(FOS)/Orafti  HP(Inulin)

What form of the nutrient was spiked into the product? What kind of solution or solvent was used to deliver the spike to the product? What percentage of the spiked sample was the spiked was the spike blanc? (How much was product diluted by spike?)

product itself no dilution

SPIFAN ERP Checklist v1.0