ERP Micro December 2019

B astin et al . : J ournal of AOAC I nternational V ol . 102, N o . 5, 2019  1599

Table 2017.09A. Summary of results for the identification of Salmonella (inclusivity)

MALDI Biotyper correctly identified

Ref. correctly identified

MALDI Biotyper incorrectly identified

Ref. incorrectly identified

MALDI Biotyper not tested

MALDI Biotyper total

Ref. not tested

Ref. total

Organism a

Salmonella Cerro Ad1173 Salmonella Enteritidis Ad2539 Salmonella Newport Ad540 Salmonella Stourbridge Ad2297 Salmonella Agona A00V038 Salmonella Dublin Ad1336 Salmonella Panama Ad1733 Salmonella Typhimurium Ad1484 Salmonella Brandenburg Ad351 Salmonella Infantis Ad2646 Salmonella Napoli Ad928 Salmonella Saintpaul A00C002 Salmonella Derby Ad1337 Salmonella Kentucky Ad1756 Salmonella Virchow F276 Salmonella Montevideo Ad912

45 45 44 45 45 45 45 45 45 45 45 45 45 45 45 45

15 15 15 15 15 15 15 15 15 15 15 14 15 15 14 15

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1

0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1

45 45 44 45 45 45 45 45 45 45 45 45 45 45 45 45

15 15 15 15 15 15 15 15 15 15 15 15 15 15 14 15

1 b

719 c

239 d

Total isolates

719

238

a  ADRIA Développement collection, Quimper, France. b  Not tested on RAPID’ Salmonella . c  The total numbers represent isolates analyzed on the three recommended culture media: Tryptic Soy Agar, Xylose Lysine Deoxycholate, and RAPID’ Salmonella . d  Reference method performed from the nonselective Tryptic Soy Agar plates only.

( h ) User Manual microflex LT/SH Smart. ( i ) User Manual microflex.

See Tables 2017.09K–T for detailed results of the interlaboratory study. Laser class. —The MALDI Biotyper System with a closed safety cover is a Class 1 Laser product (exempt from all beam hazard control measures). With the safety cover opened, it becomes a Class 4 Laser product (considered to be hazardous to the skin and eyes from direct and scattered radiation). It has been designed and tested in accordance with International Electrotechnical Commission Publication 61010-1 (Pollution Degree 2), Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use (13). The procedure for cleaning the reusable polished stainless- steel target plates with the trifluoroacetic acid should be conducted in a chemical fume hood. Do not use the MALDI Biotyper System until it has been installed by a Bruker service representative and laboratory staff have been appropriately trained by a Bruker representative. Read the user manual for the microflex LT/SH smart (No. 605415) or the microflex (No. 1831173) for operational and safety instructions before using the MALDI Biotyper System. Please refer to all user manuals listed below for the complete safety precautions: ( a ) Instructions for Use MBT Galaxy HCCA Matrix GPR when using the Galaxy to support the sample preparation. ( b ) Instructions for Use MBT Biotarget 96. ( c ) Instruct Ions for USE Bruker Matrix HCCA. ( d ) Instructions for Use Bruker BTS. ( e ) User Manual MBT Subtyping Module. ( f ) User Manual MBT Compass. ( g ) User Manual MBT Explorer Module.

The user should read, understand and follow all safety information in the instructions for the Bruker method. Retain the safety instructions for future reference. To reduce the risks associated with exposure to chemicals and biohazards, perform microbiological testing in a properly equipped laboratory under the control of trained personnel. Always follow standard laboratory safety practices, including wearing appropriate protective apparel and eye protection while handling chemicals, reagents, and contaminated samples. Avoid contact with the isolates and reagents. Dispose of isolates, chemicals, and reagents according to current local, regional, and national guidelines. The Bruker MALDI Biotyper can detect biosafety level 2/3 organisms. Biological samples have the potential to transmit infectious diseases. Follow all applicable local, state/provincial, and/or national regulations on the disposal of biological wastes. Wear appropriate protective equipment, which includes but is not limited to protective eyewear, a face shield, clothing/ laboratory coat, and gloves. All work should be conducted in properly equipped facilities using the appropriate safety equipment (e.g., physical containment devices). Individuals should be trained in accordance with applicable regulatory and company/institution requirements before working with potentially infectious materials. The cleaning of the target plates should be conducted in a chemical fume hood. When testing foodborne bacterial isolates, a risk assessment should be performed for any new tests to determine the safety of the protocols and whether this procedure should be performed in a biological safety cabinet. In addition, the