ERP Micro December 2019

1602  B astin et al . : J ournal of AOAC I nternational V ol . 102, N o . 5, 2019

Table 2017.09F. Summary of results for the confirmation of Cronobacter (inclusivity)

MALDI Biotyper correctly confirmed

Ref. correctly confirmed

MALDI Biotyper incorrectly confirmed

Selective media no growth

MALDI Biotyper total

Ref. incorrectly confirmed

Ref. total

Organism

Cronobacter condimenti QL17031.1 a Cronobacter dublinensis QL17031.2 Cronobacter dublinensis CCUG 58094 b Cronobacter malonaticus QL123015.1A Cronobacter malonaticus CCUG 28859 Cronobacter muytjensii ATCC 51329 c Cronobacter sakazakii CCUG 21205 Cronobacter sakazakii CCUG 28857 Cronobacter sakazakii CCUG 28867 Cronobacter sakazakii CCUG 28870 Cronobacter sakazakii FSL F6-023 d Cronobacter sakazakii FSL F6-032 Cronobacter sakazakii FSL F6-038 Cronobacter sakazakii FSL F6-041 Cronobacter sakazakii FSL F6-046 Cronobacter sakazakii FSL F6-051

41 42 42 42 42 42 42 42 42 42 42 42 42 42 42 42

14 14 14 12 14 13 14 14 13 12 11 14 14 14 13 14

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 2 0 1 0 0 1 2 3 0 0 0 1 0

1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

41 42 42 42 42 42 42 42 42 42 42 42 42 42 42 42

14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14

1 e

671 f

224 g

Total isolates

671

214

10

a  QL = Q Laboratories collection (Cincinnati, OH). b  CCUG = Culture Collection University of Gothenburg (Gothenburg, Sweden). c  ATCC = American Type Culture Collection biological materials resource (Manassas, VA). d  FSL = Collection of the Food Safety Laboratory, Cornell University (Ithaca, NY). e  No growth on CCI only. f  The total numbers represent isolates analyzed on the three recommended culture media: Tryptic Soy Agar, Cronobacter Chromogenic Isolation Agar, and Enterobacter sakazakii Isolation Agar. g  Reference method performed from Tryptic Soy Agar only.

Table 2017.09G. Summary of results for the identification of Gram-negative organisms ( Cronobacter exclusivity)

MALDI Biotyper incorrectly identified

Ref. incorrectly identified

Selective media no growth

MALDI Biotyper correctly identified

Ref. correctly identified

MALDI Biotyper total

Ref. total

Organism

Citrobacter farmeri ATCC 51633 a Citrobacter freundii QL11007.10 b Escherichia coli ATCC 10536

42 40 42 42 36 42 37 41

12 14 13 14 14 10 14 14

0 0 0 0 0 0 0 1 1

2 0 1 0 0 4 0 0 7

0 2 0 0 6 0 5 0

42 40 42 42 36 42 37 42

14 14 14 14 14 14 14 14

Hafnia alvei ATCC 51815

Klebsiella pneumoniae ATCC 10031 Pseudomonas aeruginosa ATCC 35032

Salmonella Hadar ATCC 51956 Serratia marcescens QL11007.1

323 c

112 d

Total isolates

322

105

13

a  ATCC = American Type Culture Collection biological materials resource (Manassas, VA). b  QL = Q Laboratories collection (Cincinnati, OH). c  The total numbers represent isolates analyzed on the three recommended culture media: Tryptic Soy Agar, Cronobacter Chromogenic Isolation Agar, and Enterobacter sakazakii Isolation Agar. d  Reference method performed from Tryptic Soy Agar plates only.