ERP Micro December 2019

1610  B astin et al . : J ournal of AOAC I nternational V ol . 102, N o . 5, 2019

Table 2017.09T. Comparative results for the confirmation and identification of non- Campylobacter Gram-negative organisms (exclusivity)

Modified Charcoal Cefoperazone Deoxycholate

RAPID’ Campylobacter Agar

Columbia Blood Agar Ref. Columbia Blood Agar  a N  b X  c Percent PA d N X Percent PA N X Percent PA N X Percent PA N X Percent PA Campy Cefex Agar

Target

Collaborator

4 e 4 1.00 1 f 1 1.00 1 f 1 1.00 8 8 1.00 6 g 6 1.00 2 h 2 1.00 4 e 4 1.00 8 8 1.00 0 i 0 1.00 0 i 0 1.00 0 i 0 1.00 8 8 1.00 7 j 7 1.00 1 f 1 1.00 7 j 7 1.00 8 8 1.00 7 j 7 1.00 1 f 1 1.00 7 j 7 1.00 8 8 1.00 7 j 7 1.00 2 h 2 1.00 7 j 7 1.00 8 8 1.00 7 j 7 1.00 2 h 2 1.00 6 g 6 1.00 8 8 1.00 4 e 4 1.00 1 f 1 1.00 3 k 3 1.00 8 8 1.00 6 g 6 1.00 3 k 3 1.00 4 e 4 1.00 8 8 1.00 4 e 4 1.00 3 k 3 1.00 5 l 5 1.00 8 8 1.00 7 j 7 1.00 0 i 0 1.00 0 i 0 1.00 8 8 1.00 6 g 6 1.00 1 f 1 1.00 4 e 4 1.00 8 8 1.00 4 e 4 1.00 0 i 0 1.00 1 f 1 1.00 8 8 1.00 6 g 6 1.00 0 i 0 1.00 0 i 0 1.00 8 8 1.00 5 l 5 1.00 0 i 0 1.00 2 h 2 1.00 8 8 1.00 2 h 2 1.00 7 j 7 1.00 8 8 1.00 NA 0 i 0 1.00 6 g 6 1.00 8 8 1.00 nt nt NA n

Non- Campylobacter Gram-negative organisms

1 2 3 4 5 6 7 8 9

8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8

1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

10 11 12 13 14 15 16 17

1.00 nt m nt

1.00

a  Reference method only confirmed to the genus level. b N = Number of correct identifications. c X = Total number of isolates tested. d  PA = Positive agreement. e  No growth observed on agar for four isolates.

f  No growth observed for seven isolates. g  No growth observed for two isolates. h  No growth observed for six isolates. i  No growth observed for all eight isolates. j  No growth observed for one isolate. k  No growth observed for five isolates. l  No growth observed for three isolates. m  nt = Data not being used for mCCDA. n  NA = Not applicable.

( 2 )  Preparation of 80% aqueous trifluoroacetic acid (conducted in a fume cabinet).—(a) Transfer 50 μL HPLC grade water into a 1.5 mL microcentrifuge tube. ( b ) Carefully add 200 μL trifluoroacetic acid. ( c ) Close the tube tightly. ( d ) Mix by inverting the tube five times. ( 3 )  Target cleaning procedure (conduct in a fume cabinet) .—( a ) Transfer the target into a suitable container, e.g., a 100 mm glass Petri dish, and pour in enough 70% aqueous ethanol (prepared as described above) to cover the target plate surface. ( b ) Incubate for 5 min at room temperature (20–25°C). ( c ) Remove the target plate and rinse it thoroughly under running tap water. ( d ) Using a fiber-free cloth, clean the target plate thoroughly with 70% aqueous ethanol.

efficient cleaning of the reusable polished stainless-steel target plates, one using trifluoracetic acid and one using GdnHCl. ( a )  Trifluoroacetic acid procedure .—Conducted in a chemical fume hood. Before each run, ensure that the target plate was cleaned properly. Please prepare the solutions required for cleaning targets as follows.—( 1 )  Preparation of 70% aqueous ethanol .— ( a ) To prepare 100 mL solution, measure 30 mL HPLC grade water with a graduated cylinder. ( b ) Transfer the water into a beaker. ( c ) Measure 70 mL absolute ethanol and mix with the water in a beaker. ( d ) Generate a homogeneous mixture by transferring the mixture from the beaker into the graduated cylinder and back again. ( e ) Repeat step ( d ) five times.