ERP Micro December 2019
B astin et al . : J ournal of AOAC I nternational V ol . 101, N o . 5, 2018 1613
Table 2017.10A. Summary of results for the confirmation and identification of L. monocytogenes : inclusivity
MALDI Biotyper correctly identified to species level
MALDI Biotyper correctly
Ref. correctly identified to species level
Ref. correctly
MALDI Biotyper incorrectly identified
MALDI Biotyper not tested
MALDI Biotyper total
identified to genus level
identified to genus level
Ref. not tested
Organism
Ref. total
62
62
14
16
0
0
2
62
16
L. monocytogenes Ad32.183 L. monocytogenes Ad285 L. monocytogenes Ad1494 L. monocytogenes Ad534 L. monocytogenes Ad273 L. monocytogenes Ad494 L. monocytogenes A00C042 L. monocytogenes Ad1781 L. monocytogenes Ad626 L. monocytogenes Ad12407/3139 L. monocytogenes Ad270 L. monocytogenes Ad1498 L. monocytogenes Ad253 L. monocytogenes Ad1679 L. monocytogenes Ad816 L. monocytogenes Ad618
62
62
14
16
0
0
2
62
16
61
61
14
16
0
0
3
61
16
62
62
14
16
0
0
2
62
16
60
61
14
16
1
0
2
62
16
62
62
14
16
0
0
2
62
16
60
61
14
16
0
0
3
61
16
62
62
14
16
0
0
2
62
16
61
62
14
16
0
0
2
62
16
62
62
14
16
0
0
2
62
16
62
62
13
16
0
0
2
62
16
62
62
14
16
0
0
2
62
16
62
62
13
16
0
0
2
62
16
62
62
14
16
0
0
2
62
16
62
62
14
16
0
0
2
62
16
61
62
15
16
0
0
2
62
16
989 a
990 b
256 c
223
256
1
0
34
Total isolates
985
a MALDI Biotyper result identified to a genus level. b The total numbers represent isolates analyzed on the four recommended culture medias: TSA/YE, O&A, OXA, and RAPID’L.mono. c Reference method performed from TSA/YE agar only.
Read the User Manual for the microflex LT/SH smart (No. 605415) or the microflex (No. 1831173) for operational and safety instructions before using the MALDI Biotyper System. Please refer to all User Manuals listed below for the complete safety precautions. ( a ) Instructions for Use MBT Galaxy HCCA Matrix GPR when using the Galaxy to support the sample preparation. ( b ) Instructions for Use MBT Biotarget 96. ( c ) Instructions for USE Bruker Matrix HCCA. ( d ) Instructions for Use Bruker Bacterial Test Standard. ( e ) User Manual MBT Subtyping Module. ( f ) User Manual MBT Compass.
C. General Instructions
Follow all instructions carefully. Failure to do so may lead to inaccurate results. Caution: Laser class.— The MALDI Biotyper System with a closed safety cover is a Class 1 Laser (exempt from all beam hazard control measures) product. With the safety cover opened it becomes a Class 4 Laser (considered to be hazardous to the skin and eyes from direct and scattered radiation) product. It has been designed and tested in accordance with International Electrotechnical Commission Publication 1010-1 (Pollution Degree 2) Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use . Do not use the MALDI Biotyper System until it has been installed by a Bruker service representative and laboratory staff have been appropriately trained by a Bruker representative.
( g ) User Manual MBT Explorer Module. ( h ) User Manual microflex LT/SH Smart. ( i ) User Manual microflex.
The user should read, understand, and follow all safety information in the instructions for the Bruker method. Retain
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