ERP Micro December 2019
1614 B astin et al . : J ournal of AOAC I nternational V ol . 101, N o . 5, 2018
Table 2017.10B. Summary of results for the confirmation and identification of Listeria species (non- monocytogenes ): inclusivity
MALDI Biotyper correctly identified to species level
MALDI Biotyper correctly
Ref. correctly identified species level
Ref. correctly
MALDI Biotyper incorrectly identified
MALDI Biotyper not tested
MALDI Biotyper total
identified to genus level
identified to genus level
Ref. not tested
Organism
Ref. total
L. ivanovii Ad1737 L. welshimeri Ad1175 L. grayi Ad1504 L. innocua Ad1675 L. ivanovii Ad1308 L. ivanovii Ad1768 L. innocua Ad1676 L. welshimeri Ad1204 L. welshimeri Ad1211 L. seeligeri Ad2634
60 61 62 60 60 59 60 61 62 59 62 60
61 61 62 62 62 62 62 62 62 62 62 62
15 15 16 14 16 12 12 16 16
16 16 16 16 16 16 16 16 16 16 16 16
0 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 0 0
3 3 2 2 2 2 2 2 2 2 2 2
61 61 62 62 62 62 62 62 62 62 62 62
16 16 16 16 16 16 16 16 16 16 16 16
9
L. grayi Ad2064
15 10
L. seeligeri Ad2637
742 a
166 b
192 c
26
742
192
Total isolates
726
a MALDI Biotyper result identified to a genus level. b The total number identified to the species level by the reference method from TSA/YE. c The total number identified to the genus level by the reference method from TSA/YE.
Table 2017.10C. Comparative results for the confirmation and identification of L. monocytogenes
Candidate Tryptic Soy Agar with Yeast Ref. Tryptic Soy Agar with Yeast X a N b Percent PA c X N Percent PA X N Percent PA X N Percent PA X N Percent PA Candidate Ottaviani and Agosti Agar Candidate Oxford Agar Candidate Rapid’L. mono Agar
Target
Collaborator
1 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 2 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 3 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 4 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 5 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 6 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 16 d 16 100.0 7 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 8 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 9 16 16 100.0 16 16 100.0 0 0 e 100.0 16 f 16 100.0 16 16 100.0 10 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 16 f 16 100.0 11 15 16 93.8 16 16 100.0 16 f 16 100.0 16 16 100.0 16 g 16 100.0 12 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 13 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 14 15 15 h 100.0 16 f 16 100.0 0 0 e 100.0 15 15 h 100.0 16 i 16 100.0 15 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 16 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0 16 16 100.0
L. monocytogenes
a X=Number of correct identifications. b N=Total number of isolates tested. c PA=Positive agreement. d Collaborator confirmed 3 isolates to the species level and 16 isolates to the genus level. e Collaborator did not receive OXA. f Collaborator confirmed 15 isolates to the species level and 1 to the genus level. g Collaborator confirmed 13 isolates to the species level and 16 isolates to the genus level. h No growth observed on agar for one isolate. i Collaborator confirmed all 16 isolates to the genus level.
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