AOAC-RI ERP Book Micro Jan 19 2017

OMAMAN-35 B: Collaborative Study Protocol ERP USE ONLY January 2017

3M MDA2 E. coli O157:H7 Collaborative Study

May 2016

OMA-2016-May-XXXX

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2.3. For all test portions, follow the isolation procedure using Dynal #710.04 E.coli O157:H7 2 2.4. Prepare the beads as described by USDA/FSIS, spread plate dilutions of the bead suspension 3 (with and without acid treatment) onto modfied Rainbow® Agar. Incubate for 20-24 h at 4 35  2  C. 5 2.5. Confirm a minimum of 1 typical colony from all potential positive test portions with typical 6 colonies following the identification and confirmation procedures as per USDA FSIS. 9 10 All results are to be recorded on the data sheets provided. For each test portion, record the results 11 reported by the 3M method along with the confirmation results for both the 3M MDA2 - E. coli O157 12 (including H7) and reference methods. Immediately upon completion of each food type, the data sheet 13 should be faxed or emailed to TBD, co-Study Director. 14 immunomagnetic beads as described in USDA/FSIS. 7 8 Final Results

AOAC Research Institute Expert Review Panel Use Only

DRAFT DOCUMENT

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