AOAC-RI ERP Book Micro Jan 19 2017

OMAMAN-35 C : Instructions For Use ERP Use Only January 2017

Table 2. General enrichment protocols

Enrichment Temperature (±1°C)

Enrichment Time (hour)

Sample Size

Enrichment Broth Volume

Sample Matrix (a)

Raw beef including ground/mince and trim

975 mL BPW ISO (pre-warmed) 225 mL BPW ISO (pre-warmed) 450 mL BPW ISO (pre-warmed)

325 g

41.5

10-18

Raw meats including raw beef, pork, poultry, lamb, bison

25 g

41.5

8-18

Leafy produce (b)

200 g

41.5

18-24

Other foods including fruit (b) , vegetables, fruit/vegetable juices, fresh herbs, raw seafood, raw eggs, raw milk, cookie dough, processed meats Walnuts or nut mixes containing walnuts (this protocol is appropriate for other nuts including pecans, almonds, pistachios, cashews, chestnuts,

225 mL BPW ISO (pre-warmed)

25 g

41.5

18-24

225 mL reconstituted non-fat dry milk

25 g

41.5

18-24

(a) Frozen samples should be equilibrated to 4-8°C before addition to enrichment broth. (b) Leafy produce and fruit samples should be gently agitated by hand for 5 minutes. Do not blend or stomach. Table 3. Enrichment protocols using pre-warmed BPW ISO at 41.5 ± 1°C according to AOAC ® Official Methods SM 2016.xxx

Formatted: Not Superscript/ Subscript

Enrichment Time (hour)

Sample Size

Enrichment Broth Volume

Homogenized

Sample Matrix

Raw ground beef (73% lean)

325 g

975 mL

10-18

Manually by hand

200 g AOAC Research Institute Expert Review Panel Use Only 18-24 Gently agitated by hand for 5 minutes 25 g 225 mL 18-24 Stomach 25 g 225 mL 18-24 450 mL

Raw bagged spinach (a)

Fresh sprouts

Frozen blueberries (a)(b) Gently agitated by hand for 5 minutes (a) Leafy produce and fruit samples should be gently agitated by hand for 5 minutes. Do not blend or stomach. (b) Frozen samples should be equilibrated to 4-8°C before addition to enrichment broth.

NOTES: The recommended protocol interruption points are after enrichment or after sample lysis. Enrichment broth or sample lysate can be stored at 2-8 ° C for up to 72 hours. After removing the enrichment broth

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