AOAC-RI ERP Book Micro Jan 19 2017

4.0 DISCUSSION

The purpose of this study was to validate the 3M TM MDA for detection of E. coli O157:H7 in frozen blueberries. This study followed the format for the validation of a candidate method using the experimental setup and Probability of Detection (POD) statistical analysis provided in the February 24, 2012 version of the AOAC International Methods Committee Guidelines for Validation of Microbiological Methods for Food and Environmental Surfaces document (1). Overall, based on the evidence presented here, the 3M TM MDA is a valid method for the detection of E. coli O157:H7 in frozen blueberries. The POD statistical analysis of the results for the candidate method and the reference method identified that these methods are not significantly different from each other. Additionally, no false positives or false negatives were identified by comparing the ‘ screening stage ’ and the ‘ confirmation stage ’ of the candidate method. This validation is accurate for the target sample size (25 g) tested. 5.0REFERENCES 1. Brunelle S, LaBudde R, Nelson M, Wehling P . 2012. AOAC International Methods Committee Guidelines for Validation of Microbiological Methods for Food and Environ-mental Surfaces. Available at: http://www.aoac.org/imis15_prod/AOAC_Docs/StandardsDevelopment/AOAC _Validation_Guidelines_for_Food_Microbiology-Prepub_version.pdf. Accessed: 11/30/15. 2. Feng P, Weagant SD, Jinneman, K. 2011. U.S. Food and Drug Administration Bacteriological Analytical Manual, Chapter 4A: Diarrheagenic Escherichia coli . Available at: http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm0700 80.htm. Accessed: 11/30/15.

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