AOAC-RI ERP Book Micro Jan 19 2017

Table 6. Candidate Method Testing Results for Sprouts Matrix ‘Screening Stage’ and ‘Confirmation Stage’ (25 g Sprouts Analyzed via the 3M TM MDA)

3M MDS 18 h

Sample Number

Inoculum Level

CT-SMAC #1 CT-SMAC #2

Rainbow

O Serology H Serology

VITEK Confirmation

= + = = = + + + = = = + + = + + = = + + + = + + + = = = = =

= + = = = + + + = = = + + = + + = = + + + = + + + = = = = =

= + = = = + + + = = = + + = + + = = + + + = + + + = = = = =

= + = = = + + + = = = + + = + + = = + + + = + + + = = = = =

= + = = = + + + = = = + + = + + = = + + + = + + + = = = = =

= + = = = + + + = = = + + = + + = = + + + = + + + = = = = =

1 2 3 4 5 6 7 8 9

LOW LOW LOW LOW LOW LOW LOW LOW LOW LOW LOW LOW LOW LOW LOW LOW LOW LOW LOW LOW HIGH HIGH HIGH HIGH HIGH

+

E. coli O157

+ + +

E. coli O157 E. coli O157 E. coli O157

10 11 12 13 14 15 16 17 18 19 20

+ + + + + + + + + +

E. coli O157 E. coli O157 E. coli O157 E. coli O157 E. coli O157 E. coli O157 E. coli O157 E. coli O157 E. coli O157 E. coli O157

1 2 3 4 5 1 2 3 4 5

UNINOC UNINOC UNINOC UNINOC UNINOC

CONFIDENTIAL INFORMATION Page 11 of 17