AOAC-RI ERP Book Micro Jan 19 2017

Independent Laboratory Evaluation of the 3M™ Molecular Detection Assay 2 - E. coli O157:H7 for the Detection of E. coli O157:H7 in Raw Ground Beef

The 3M ™ Molecular Detection Assay 2 (MDA 2) – E. coli O157 (including H7) employs real-time isothermal technology for the rapid and accurate detection of E. coli O157 (including H7) from select food matrices. This study included a method comparison evaluation of the 3M MDA 2 – E. coli O157:H7 to the USDA/FSIS-MLG 5.09 Detection, Isolation and Identification of Escherichia coli O157:H7 from Meat Products and Carcass and Environmental Sponges for the detection of E. coli O157:H7 in raw ground beef (73% lean). The 3M MDA 2 – E. coli O157:H7 was evaluated for its ability to accurately detect E. coli O157:H7 from raw ground beef after 10 hours of incubation. In the method comparison study, the 3M ™ MDA 2 - E. coli O157:H7 demonstrated no statistically significant differences between presumptive and confirmed results (dPOD CP ) or between candidate and reference method results (dPOD C ) for the evaluation of the raw ground beef matrix. Study Report Authors Alison DeShields, Benjamin Bastin, Patrick Bird, M. Joseph Benzinger, Jr., Erin Crowley, James Agin, and David Goins Q Laboratories, Inc., Cincinnati, OH, USA 45214 Submitting Company 3M ™ Food Safety Department 3M ™ Center

Building 260-6B-01 St. Paul, MN 55144 Independent Laboratory

Q Laboratories, Inc. 1400 Harrison Ave Cincinnati, OH 45214 United States Scope of Methods

(a) Target organism – E. coli O157 (b) Matrix

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