AOAC-RI ERP Book Micro Jan 19 2017

Materials and Methods Test Kit Information

(a) MDA 2 – E. coli O157 Test Kits (b) MDA 2 Software (c) 3M BPW ISO Formulation (d) Matrix Control Kits Additional Supplies and Reagents

(a) 3M Molecular Detection System (instrument) (b) 3M Molecular Detection Speed Loader Tray (c) 3M Molecular Detection Heat Block Insert (d) 3M Molecular Detection Cap/Decap Tool [Reagent] (e) 3M Molecular Detection Cap/Decap Tool [Lysis] (f) Buffered Peptone Water, ISO formulation (ISO BPW)

Apparatus

(a) Top Loading Balance – capable of weighing and measuring 25 – 2000 grams. (b) Filter laboratory blender bags. (c) Laboratory paddle blender - Seward 3500 or equivalent: for sample homogenization. (d) Incubators – capable of maintaining 41.5 ± 1 °C (e) Dry bath incubators – capable of maintaining 100 ± 1 °C. (f) Calibrated Thermometers – capable of measuring, 41.5 ± 1 °C, and 100 ± 1 °C. (g) Serological Pipette Bulbs (Automatic Pipette) – For sampling and delivering of 1 mL - 10 mL.

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