AOAC-RI ERP Book Micro Jan 19 2017

(h) Serological pipettes – Aerosol resistant. (i) Microcentrifuge – For 1.5 mL microcentrifuge tubes. (j) Adjustable, variable volume single pipette capable of sampling and delivering 10-1000 µL. (k) Adjustable, variable volume multichannel pipette capable of sampling and delivering 30 µL. (l) Micropipette tips – Aerosol resistant.

(m) Sterile Gloves (n) Felt-tip marker.

(o) Refrigerator – Capable of maintaining 2-8 °C. (p) Freezer – Capable of maintaining -20 °C. (q) Calibrated Timer

Reference Materials Organisms used in the method comparison study were obtained from the following sources: (a) American Type Culture Collection (ATCC) — Manassas, VA. www.atcc.org.

Safety Precautions Enrichment

Shiga-toxin producing Escherichia coli is a Biosafety Level 2 organism. Biological samples such as enrichments have the potential to transmit infectious diseases. Follow all applicable local, state/provincial, and/or national regulations on disposal of biological wastes. Wear appropriate protective equipment which includes but is not limited to: protective eyewear, face shield, clothing/lab coat, and gloves. All work should be conducted in properly equipped facilities utilizing the appropriate safety equipment (for example, physical containment devices). Individuals should be trained in accordance with applicable regulatory and company/institution requirements

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