AOAC-RI ERP Book Micro Jan 19 2017

O157 and the reference method indicated that there was no significant difference at the 5% level between the number of positive results by the two methods. The POD analysis between the 3M ™ MDA 2 - E. coli O157 presumptive and confirmed results indicated that there was no significant difference at the 5% level. A summary of POD analyses [9] are presented in Tables 4-7 of the Appendix. Table C: Summary of Results

Raw Ground Beef (73% lean 27% fat)

Matrix Method Result

3M ™ MDA 2 - E. coli O157

USDA/FSIS MLG 5.09

Confirmed 10 Hours

Presumptive

Time Point Uninoculated

10 Hours

0/5

0/5

0/5

11/20

Low High

11/20

7/20

5/5

5/5

5/5

Raw Ground Beef- 10 Hour Enrichment For the low inoculation level of the 3M ™ MDA 2 - E. coli O157, there were 11 presumptive positives and 11 confirmed positives following the USDA/FSIS MLG 5B.05 reference method. There were 7 confirmed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 5.09 reference method. There were 5 confirmed positives following the reference method. For the low inoculation level, a dPOD C value of 0.20 was obtained with a 95% confidence interval of (-0.10, 0.46), indicating no statistically significant difference between the candidate and reference method. A dPOD CP value of 0.00 was obtained with a 95% confidence interval of (-0.28, 0.28), indicating no statistically significant difference between the candidate presumptive and the USDA/FSIS MLG 5.09 value of 0.00 was obtained with a 95% confidence interval of (-0.43, 0.43), indicating no statistically significant difference between the candidate and reference method. A dPOD CP value of 0.00 was obtained with a 95% confidence interval of (-0.43, 0.43), indicating no statistically significant difference between the candidate presumptive and confirmed results. Detailed results of the POD analyses are presented in Tables 5 and 7 of the Appendix. reference method confirmed results. For the high inoculation level, a dPOD C

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