AOAC-RI ERP Book Micro Jan 19 2017

Discussion The 3M ™ MDA 2 - E. coli O157 accurately detected E. coli O157:H7 from raw ground beef at 10 hour time point. The MDA 2 - E. coli offers the benefits of extremely high sensitivity and high specificity for the detection of E. coli O157 while reducing the overall time to presumptive results. The MDA 2 - E. coli O157 also reduces the total analyst time required to obtain final results by implementing a single heat lysis step and cool step, followed by a single transfer to molecular reaction tubes. The small foot print of the Molecular Detection System requires minimal space, making it easy to move the instrument or pair additional MDS units to perform multiple MDA runs concurrently. The updated software is user friendly with easy to interpret results. Also, the ability to examine Relative Light Unit (RLU) curves makes troubleshooting more streamline if problems occur during testing. There was no significant, statistical difference observed at the 10 hour time point for the evaluation of raw ground beef. Conclusion The data from the study, within the statistical uncertainty, support the product claims of the 3M ™ MDA 2 - E. coli O157 in the selected food matrix. The results obtained by the POD analysis of the method comparison study demonstrated that there were no statistically significant differences between the number of positive samples detected by the candidate and the reference method for the raw ground beef food matrix for 10 hour time point.

References [1] U.S. Department of Agriculture Food Safety and Inspection Service Microbiology Laboratory Guidebook, Revision 5.09 Isolation and Identification of Escherichia

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