AOAC-RI ERP Book Micro Jan 19 2017

3.2 x 10 3

Raw Ground Beef

0/5

1 APC conducted in accordance with FDA/BAM Chapter 3 2 E. coli O157:H7 screen conducted using USDA/FSIS MLG 5.09 lateral flow device. Table 2: MPN Summary Table for Raw Ground Beef

Raw Ground Beef (73% lean, 27% fat) E. coli O157:H7 ATCC 43895 Low Level Inoculum (0.2-2 MPN/Test Portion) A B C

D +

E + +

5 x 650 g 5 x 130 g

- -

-

+

+

-

-

20 x 325 g (Reference Samples)

7/20 0.49 0.25

MPN/Test portion

Low Conf. Limit MPN/Test Portion High Conf. Limit MPN/Test Portion

0.85 High Level Inoculum (2-5 MPN/Test Portion) A B C

D

E

5 x 130 g 5 x 65 g

+ +

+

+ +

+ +

+

-

-

5 x 325 g (Reference Samples)

5/5

MPN/Test portion

2.97 1.30

Low Conf. Limit MPN/Test Portion High Conf. Limit MPN/Test Portion

6.81 * MPN was calculated for the low level inoculation for raw ground beef using five 650 g, five 130 g and the 20 low level reference method samples (325 g) using the LCF MPN Calculator version 1.6 provided by AOAC-RI http://www.lcfltd.com/customer/LCFMPNCalculator * MPN was calculated for the high level inoculation for raw ground beef using five 130 g, five 65 g and the 5 high level reference method samples (325 g) using the LCF MPN Calculator version 1.6 provided by AOAC-RI http://www.lcfltd.com/customer/LCFMPNCalculator

Table 3: Detailed Results for the 3M™ MDA 2 - E. coli O157 in Raw Ground Beef (73% Lean)

Raw Ground Beef E. coli O157:H7 ATCC 43895

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