AOAC-RI ERP Book Micro Jan 19 2017

Overall, based on the results of this study, the 3M™ Molecular Detection Assay (MDA) is a valid method for the detection of Escherichia coli O157:H7 in 325 g ground beef (18 hr enrichment). The candidate methods explored here with the targeted sample sizes produce equivalent results in both the ‘screening stage’ and the ‘confirmation stage’ and produce equivalent results when compared with the USDA MLG reference method, typically employed for ground beef. 1. Brunelle S, LaBudde R, Nelson M, Wehling P . 2012. AOAC International Methods Committee Guidelines for Validation of Microbiological Methods for Food and Environ-mental Surfaces. Available at: http://www.aoac.org/imis15_prod/AOAC_Docs/StandardsDevelopment/AOAC _Validation_Guidelines_for_Food_Microbiology-Prepub_version.pdf. Accessed: 11/30/15. 2. United States Department of Agriculture, Food Safety Inspection Service, Office of Public Health Science . 2014. Microbiology Laboratory Guidebook Method 5.09: Detection, Isolation and Identification of Escherichia coli O157:H7 from Meat Product and Carcass and Environmental Sponges. Available at: http://www.fsis.usda.gov/wps/wcm/connect/51507fdb-dded-47f7- 862d-ad80c3ee1738/MLG-5pdf?MOD=AJPERES. Accessed: 11/30/2015. 6.0 DATA DISPOSITION Copies of raw data and final reports from this study will be archived at Food Safety Net Services, Ltd., 199 W. Rhapsody, San Antonio, TX 78216, and shall be kept c onfidential as per the Agreement between FSNS and 3M™. 5.0 REFERENCES

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