AOAC-RI ERP Book Micro Jan 19 2017

OMAMAN-36 E/ AOAC PTM Report ERP Use Only January 2017

Figure 5: Summary of sample prep lot variation Automated sample prep

20 25 30 35 40

Prep Lot1 Prep Lot2 Prep Lot3

Ct

10 15

0 5

Salmonella abony

Salmonella enteritidis

Citrobacter freundii

Internal control

Bacterial strain

Figure 5 : Ct values for Salmonella abony, Salmonella enteritidis, C. freundii and the internal control were not significantly different, indicating that there were no effects of the lot of the QIAsymphony Bacteria kit on the detection.

Figure 6: Rotor-Gene Q instrument variation with automated sample prep

20 25 30 35 40

Cycler 1 Cycler 2

Ct

10 15

AOAC Research Institute Expert Review Panel Use Only

0 5

Salmonella abony

Salmonella enteritidis

Citrobacter freundii

Internal control

Bacterial Strain

Figure 6 : Ct values for Salmonella abony, Salmonella enteritidis, C. freundii and the internal control were not significantly different, indicating that there was no effect of the Rotor-Gene Q instruments on the results obtained

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