AOAC-RI ERP Book Micro Jan 19 2017

OMAMAN-35 A : Collaborative Study Manuscript For ERP Use Only January 2017

(jj) 3M™ Petrifilm™ Rapid Aerobic Count – Available from 3M Food Safety (St. Paul, MN, 55144-1000,

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USA)

(kk) 3M™ Rapid Plate Spreader – Available from 3M Food Safety (St. Paul, MN, 55144-1000, USA)

C. General Instructions

(a) Store the 3M Molecular Detection Assay 2 – E. coli O157 (including H7) at 2-8°C. Do not freeze. Keep kit away from light during storage. After opening the kit, check that the foil pouch is undamaged. If the pouch is damaged, do not use. After opening, unused reagent tubes should always be stored in the re- sealable pouch with the desiccant inside to maintain stability of the lyophilized reagents. Store resealed pouches at 2-8°C for no longer than 60 days. Do not use 3M Molecular Detection Assay 2 - E. coli

O157 (including H7) past the expiration date.

(b) Follow all instructions carefully. Failure to do so may lead to inaccurate results.

Safety Precautions

The 3M MDA 2 – E. coli O157 (including H7) is intended for use in a laboratory environment by professionals trained in laboratory techniques. 3M has not documented the use of this product in industries other than the food and beverage industries. For example, 3M has not documented this product for testing drinking water, pharmaceutical, cosmetics, clinical or veterinary samples. The 3M MDA 2 – E. coli O157 (including H7) has not been evaluated with all possible food products, food

processes, testing protocols or with all possible strains of bacteria.

As with all test methods, the source of enrichment medium can influence the results. The 3M MDA 2 – E. coli O157 (including H7) has only been evaluated for use with the enrichment media specified in the

Instructions for Use section.

The 3M™ Molecular Detection instrument is intended for use with samples that have undergone heat treatment during the assay lysis step, which is designed to destroy organisms present in the sample. Samples that have not been properly heat treated during the assay lysis step may be considered a AOAC Research Institute Expert Review Panel Use Only potential biohazard and should NOT be inserted into the 3M MDS instrument. The user should read, understand and follow all safety information in the instructions for the 3M MDS and the 3M MDA 2 – E. coli O157 (including H7). Retain the safety instructions for future reference. Periodically decontaminate laboratory benches and equipment (pipettes, cap/decap tools, etc.) with a 1- 5% (v:v in water) household bleach solution or DNA removal solution. When testing is complete, follow current industry standards for the disposal of contaminated waste. Consult the Safety Data Sheet To reduce the risks associated with exposure to chemicals and biohazards: Performpathogen testing in a properly equipped laboratory under the control of trained personnel.Always follow standard laboratory safety practices, including wearing appropriate protective apparel and eye protection while handling reagents and contaminated samples.Avoid contact with the contents of the enrichment media for additional information and local regulations for disposal.

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