AOAC-RI ERP Book Micro Jan 19 2017

OMAMAN-35 A : Collaborative Study Manuscript For ERP Use Only January 2017

and reagent tubes after amplification. Dispose of enriched samples according to current industry

1 2 3 4 5 6 7 8 9 0 1 2 6 7 8 9 0 1 2 3 4

standards.

To reduce the risks associated with environmental contamination:Follow current industry standards for

disposal of contaminated waste.

D. Sample Enrichment

Foods

(a) Allow BPW ISO enrichment medium to pre-warm to 41.5±1°C. See Table A for matrix-specific

enrichment protocols.

(b) Aseptically combine the enrichment medium and sample. For all meat and highly particulate samples,

the use of filter bags is recommended.

(c) Homogenize by stomacher, hand, or gentle agitation thoroughly for 2 ± 0.2 minutes. Incubate matrices

according to the instructions provided in Table A. 3 4 E. P REPARATION OF THE 3M™M OLECULAR D ETECTION S PEED L OADER T RAY 5

(a) Wet a cloth or paper towel with a 1-5% (v:v in water) household bleach solution and wipe the 3M™

Molecular Detection Speed Loader Tray.

(b) Rinse the 3M Molecular Detection Speed Loader Tray with water. (c) Use a disposable towel to wipe the 3M Molecular Detection Speed Loader Tray dry. (d) Ensure the 3M Molecular Detection Speed Loader Tray is dry before use

Table A. 3M MDA 2 – E. coli O157 (including H7)enrichment protocolsusing pre-warmed BPW ISO

at 41.5 ± 1°Caccording to AOAC Official Method SM 2016.XX

Enrichment Time (hour)

Sample Size

Sample Matrix

Enrichment Broth Volume

Homogenized

Manually by hand

325 g

Raw ground beef (73% lean)

975 mL

10-18

200 g AOAC Research Institute Expert Review Panel Use O ly 450 mL 18-24 Gently agitated by hand for 5 minutes 25 g 225 mL 18-24 Stomach

Raw bagged spinach(a)

Fresh sprouts

Gently agitated by hand for 5 minutes

25 g

Frozen blueberries(a)(b)

225 mL

18-24

(a) Leafy produce and fruit samples should be gently agitated by hand for 5 minutes. Do not blend or stomach. 5 (b) Frozen samples should be equilibrated to 4-8°C before addition to enrichment broth. 6 7 8 9 F.P REPARATION OF THE 3M™M OLECULAR D ETECTION C HILL B LOCK I NSERT 0

7