AOAC-RI ERP Book Micro Jan 19 2017

OMAMAN-35 A : Collaborative Study Manuscript For ERP Use Only January 2017

Table 2016.1A : Summary of Results for the Detection of E. coli O157:H7 in Raw Ground Beef (325g)

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Method a

3M ™ MDA ™ 2 E. coli O157 (including H7)

Un-inoculated

Low

High

Inoculation Level

Candidate Presumptive Positive/ Total # of Samples Analyzed

0/120

32/120

97/120

0.00 (0.00, 0.03) 0.00 (0.00, 0.00) 0.00 (0.00, 0.17) 0.00 (0.00, 0.24) 0.02 (0.00, 0.06) 0.12 (0.11, 0.16) 0.04 (0.00, 0.09) 0.13 (0.12, 0.16) 0.00 (0.00, 0.03) 0.00 (0.00, 0.17) 0.00 (0.00, 0.17) 0.00 (0.00, 0.24) 0.00 (0.00, 0.03) 0.00 (0.00, 0.17) 0.00 (0.00, 0.17) 0.00 (0.14, 0.20) 1.00 2/120 0.0.031 0/120 1.00

0.27 (0.19, 0.35) 0.45 (0.40, 0.52) 0.00 (0.00, 0.16) 0.45 (0.40, 0.52) 0.26 (0.18, 0.34) 0.45 (0.40, 0.52) 0.00 (0.00, 0.13) 0.45 (0.40, 0.51) 0.24 (0.16, 0.32) 0.44 (0.39, 0.51) 0.00 (0.00, 0.13) 0.44 (0.39, 0.50) 0.35 (0.26, 0.44) 0.48 (0.42, 0.52) 0.05 (0.00, 0.23) 0.48 (0.43, 0.52) -0.11 (-0.22, 0.01) 0.01 (-0.10, 0.12) 0.760 31/120 0.920 29/120 0.908 0.342

0.81 (0.67, 0.93) 0.37 (0.33, 0.42) 0.15 (0.07, 0.32) 0.40 (0.35, 0.50) 0.80 (0.69, 0.91) 0.38 (0.34, 0.44) 0.13 (0.04, 0.29) 0.40 (0.36, 0.49) 0.77 (0.63, 0.91) 0.39 (0.34, 0.45) 0.18 (0.09, 0.37) 0.43 (0.38, 0.52) 0.79 (0.67, 0.92) 0.38 (0.34, 0.44) 0.15 (0.07, 0.33) 0.41 (0.37, 0.51) 0.005 96/120 0.018 92/120 0.002

Candidate Presumptive POD (CP)

b

s r

c

s L

d

s R

P Value e

Candidate Confirmed Positive/ Total # of Samples Analyzed

Candidate Confirmed POD (CC)

s r

s L

s R

P Value

Candidate Confirmed Positive/ Total # of Samples Analyzed Candidate Presumptive Positive that Confirmed POD (C)

s r

s L

s R

P Value

Positive Reference Samples/ Total # of Samples Analyzed AOAC Research Institute Expert Review Panel Use Only 0/120 42/120 95/120 Reference POD s r

s L

s R

P Value

1.00

0.01

-0.017 (-0.059, 0.017) -0.000 (-0.031, 0.031)

-0.025 (-0.129, 0.080)

dLPOD (Candidate vs. Reference) f

dLPOD (Candidate Presumptive vs. Candidate Confirmed) f 0.008 (-0.092, 0.109) a Results include 95% Confidence Intervals, r Repeatability Standard Deviation, c Among-Laboratory Standard Deviation, d Reproducibility Standard Deviation 2 e P Value = Homogeneity test of laboratory PODs; f A confidence interval for dLPOD that does not contain the value 0 indicates a statistical significant difference between 3 the two methods 4

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