AOAC-RI ERP Book Micro Jan 19 2017
OMAMAN-35 A : Collaborative Study Manuscript For ERP Use Only January 2017
Table 2016.2A : Comparative Results for the Detection of E. coli O157:H7 in Raw Ground Beef (73% Lean) by the 3M ™ MDA 2 – E. coli O157 (including H7) 1 Method vs. USDA/FSIS MLG Chapter 5.09 in a Collaborative Study (Un-inoculated Control) 2
Candidate presumptive (CP)
Candidate confirmed (CC)
Matrix/ Inoculation Level
Candidate result (C)
Reference method (R)
C vs. R
Statistic
Laboratory
POD (CP) 0.00 0.00 0.00 0.00 0.00 0.00 0.00 NA 0.00 0.00 0.00 0.00 0.03 0.00 0.00 0.17 0.00 0.00 0.17 0.00 0.00 0.24 1.00 NA NA NA 0.00 NA
POD (CC) 0.00 0.00
POD (C) 0.00 0.00 0.00 0.00 0.00 0.00 0.00 NA 0.00 0.00 0.00 0.00 0.03 0.00 0.00 0.17 0.00 0.00 0.17 0.00 0.00 0.24 1.00 NA NA NA 0.00 NA
POD (R) 0.00 0.00 NA 0.00 0.00 0.00 NA 0.00 0.00 NA 0.00 0.00 NA 0.00 NA 0.00 0.00 0.03 0.00 0.00 0.17 0.00 0.00 0.17 0.00 0.00 0.24
dLPOD (C,R)
dLPOD (CP,CC)
N
X
N
X
N
X
N
X
1 2 3 4 5 6 8 9
12 12 12 12 12 12 12 12 12
0 0 0 0 0 0 0 0 0
12 12
0 0 0 2 0 0 0
12 12
0 0 0 0 0 0 0 0 0
12 12 12 12 12 12 12 12 12
0 0 0 0 0 0 0
0.00 0.00 0.00 0.00 0.00 0.00 0.00 NA 0.00 -0.00 NA NA
0.00 0.00 0.00 0.00 0.00 0.00 0.00 NA 0.00 0.00 NA NA
Raw Ground Beef (73% Lean)/ Un- inoculated
NA NA
NA NA
NA
NA NA
NA NA
NA
0.00
NA
12 12 12 12 12 12
0.167
12 12 12 12 12 12
0.00
7 a
NA NA
NA NA
NA
NA NA
NA NA
0.00 0.00 NA 0.00 0.00
10 11 12 14 15 13 a
NA NA
NA NA
NA NA
NA NA
0 f
0
0 f
0
NA NA
NA NA
NA
NA NA
NA NA
NA
NA
12
0
12
0
0.00
12
0
12
0
0.00
0.00
NA NA
NA NA
NA
NA NA
NA NA
NA
NA
Estimate
All
120
0
120
2
0.02 0.00 0.06 0.12 0.11 0.17 0.04 0.00 0.09 0.13 0.12 0.16
120
0
120
0
-0.000 -0.031 0.031
0.017 -0.059 0.017
LCL UCL LCL UCL LCL UCL UCL LCL s r b s L c d
s R 0.031 1.00 a N/A – Laboratory did not participate or submit data for this matrix b Repeatability Standard Deviation, c Among-Laboratory Standard Deviation, d Reproducibility Standard Deviation, e P T Value = Homogeneity test of laboratory PODs, f Original results identified this test sample(s) as E. coli O157:H7; reversed due to 16S rRNA results, LCL – Lower Confidence Limit; UCL – Upper Confidence Limit AOAC Research Institute Expert Review Panel Use Only P T e
3 4 5
17
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