AOAC-RI ERP Book Micro Jan 19 2017

OMAMAN-35 A : Collaborative Study Manuscript For ERP Use Only January 2017

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Table 1. Individual Collaborator Results for Raw Ground Beef (73% Lean)

High Level Test Portions

Low Level Test Portions

Un-inoculated Test Portions

1

2

3

4

5

6

7

8

9 10 11 12 1

2

3

4

5

6

7

8

9 10 11 12

1

2

3

4

5

6

7

8

9 10 11 12

3M MDA 2 - E. coli O157 (including H7)

Lab a 1

+ - - + - + + + + -

-

- + + - - - - - - -

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2 + + + - b + + + + + + + + - - b - + - + - - + - - - b - - - - - - - - - - - - 3 + + + + + + + + + + + + - - + + + + + + + c + + - - - - - - - - - - + + - 4 + + + + + + + + + + + + - - - - - - - - - + - + - - - - - - - - - - - - 5 + c + + + - b - + + c + - + - b - - + - - - + - - - - - - - - - - - + c + c - - - - 6 + + + + + + + + + + + + + - - - - - + - - + + + - - - - - - - - - - - - 7 n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a 8 + + + + + + + + + - + + - - - - - - + - - + + - - - - - - - - - - - - - 9 + + + + + + - + + + + + - - - - - - - + + + - - - - - - - - - - - - - - 10 + - - - + c + c + c - b - - + - - + - - + c - - - - - - b - - - - - - - - - - - - - 11 + + + c - - - + + - b + + c + + + - - - - - - + - b - - - d - - - - - - - - - - - 12 + + + - + + + - + - + + - - + - - - - + + + - - - - - - - - - - - - - - 13 n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a 14 + - - - b + + + + + + + - + - + - - + c - - - - + c - - - - - - - - - - - - - 15 n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a USDA/FSIS – MLG 5.09 Lab a 1 + + + - + + + - + - + - - - - + + - - - - - - - - - - - - - - - - - - - 2 + - + + + + + + + + + + - + + - - - + - - + - + - - - - - - - - - - - - 3 + + + + + + + + + + + + - - + + - + + + - - + + - - - - - - + - - - - - 4 + + + + + - - + - + + + + + - - - - + - + - - - - - - - - - - - - - - - 5 + - + + + + + + + + + - - - - - - - + + + - - + - - - - - - - - - - - - 6 + + + + + + + + + + - + + + - + - - - + + + + - - - - - - - - - - - - - 7 n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a 8 + + + + + + + + + + + + - - - - - - - - - - + - - - - - - - - - - - - - 9 + + - + + + + + + + + + + - - - - - - + - - + + - - - - - - - - - - - - 10 - + - - - + - - - - + - + - - - - - - + - - - + - - - - - - + - - + - - 11 + + - - + + + + + + + + + + + - - - - + - - - - - - - - - - - - - - - - 12 + - - + - - + + - - - - - - - + - + - + + - + - - - - - - - - - d - - - d - 13 n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a 14 + + - + - + - + + + + + + + + - + + + - - - - - - - - - - - - - - - - - 15 n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a a += E. coli O157 were detected in samples; -= E. coli O157 (including H7) were not detected in sample; n/a- lab did not participate or submit data for the matrix; b Sample was presumptive positive on 3M MDA 2 – E. coli O157 (including H7) but confirmed negative indicating a false positive result; c Sample was presumptive negative on 3M MDA 2 - E. coli O157 (including H7) but confirmed positive indicating a false negative result; d Original results were positive, revised after 16S rRNA AOAC Research Institute Expert Review Panel Use Only

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