AOAC SPIFAN Stakeholder Panel Meeting Book (September 7, 2019)

Composition of Milk

Compositionof human milk

Compositionof Bovine milk

Protein(34 g/L)

Lactose (68 g/L)

Protein(10 g/L)

Fat(40g/L)

Lactose (48 g/L)

Fat(39 g/L)

O S (13 g/L)

O S (<0.1 g/L)

Kunz,C., et.al. (1999) Clin.Perinatol. 26 :307-333 Coppa,G.V., et.al. (1993) Pediatrics 91 : 637-641

Sundekilde,U.K., et.al. (2012) J.Agric.Food Chem. 60 : 6188-6196 Mehra,R. et.al. (2006) Int.Dairy J. 16 : 1334-1340

Received from S. Austin

Human Milk Oligosaccharides

Biosynthetic Blueprint 1

A

B

Building blocks: Glc, Gal, GlcNAc, Fuc, Neu5Ac

Reducing end lactose is:

A: - Fucosylated - Sialylated

B: Elongated with - lacto- N -biose - N -acetyllactosamine

Further elongation, branching, fucosylation and/or sialylation possible. Formation of complex structures with high number of monosaccharides (e.g. in Tetra-F-LND II with 14 moieties ) 2 or longer DP (e.g. Tetra-F-para -LNO with DP9 ) 2

1 Triantis et.al. (2018) Frontiers in Pediatrics 6 : Article 190 2 Urashima et.al. (2011) Milk Oligosaccharides, Chapter III p. 27