AOAC SPIFAN Stakeholder Panel Meeting Book (September 7, 2019)

Analytical Techniques: 2D Electrophoresis

Gel electrophoresis • Separate out proteins by molecular weight • Multiple gel varieties: Agarose, polyacrylamide, others (intact or denatured)

• SDS-PAGE: Most commonly used • PAGE: Polyacrylamide Gel • SDS: Sodium Dodecyl Sulfate • Denaturant

• (#2 Most cited journal article)

Additional processing possible • Process bands in-tact • Digestion of gel bands • Typically LCMS work

File:Protein-SDS interaction.png. (2018, October 25). Wikimedia Commons, the free media repository . Retrieved 19:51, August 29, 2019 from https://commons.wikimedia.org/w/index.php?title=File:Protein-SDS_interaction.png&oldid=325431210 . By Jennifer0328 - Own work, CC BY-SA 4.0, https://commons.wikimedia.org/w/index.php?curid=59079152

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