AOAC SPIFAN Stakeholder Panel Meeting Book (September 7, 2019)

Analytical Techniques: LC-Mass Spectrometry (LCMS)

LCMS: Intact protein quant

X Need HRMS due to large size of the molecule (>78 kDa)

X Most triple quadrupole instruments <1400-2000 Da

TOF, Orbitrap, etc

X

Multiple charge states

X

X Lactoferrin intact will exist in multiple charge states (mass/charge = m/z)

Requires deconvolution

X

Post Translational Modifications (PTMs)

X

Glycosylation state well documented

X

Other modifications possible

X

X Instrumentation cost high for all LCMS analysis, especially HRMS