AOAC SPIFAN Stakeholder Panel Meeting Book (September 7, 2019)

LCMSMS: Surrogate Peptide Quant

X Identify surrogate peptides from protein sequence X Choose enzyme, in-silico digest X Trypsin: carboxyl side of lysine/arginine X Custom-synthesized peptides X Light = unlabeled X Heavy = Stable Isotope Labled (SIL) X Advantages of LCMS detection X Specificity, sensitivity, speed

https://www.uniprot.org/uniprot/B9VPZ5

Denature, Digest

Measure SFQLFGSPPGQR, GSNFQLDQLQGR

Analytical Techniques Summary