July 2019 Sugar ERP - Review Book

AOAC SMPR ERPs - 2019 METHOD REVIEW FORM

Submission Date

2019-07-04 08:23:21

Name

Martine van Gool

E-mail

martine.vangool@frieslandcampina.com

Organization

FrieslandCampina

Title of Method

Determination of sugars in animal feed, pet food and human food applying ion chromatography with pulsed amperometric detection (IC-PAD): Single lab validation

AOAC Candidate Method Number (e.g. ALN-01)

SUG-005

Applicable SMPR

SMPR 2018.001

I. Summary of the Method

The method determines the quantity of fructose, galactose, glucose, sucrose, maltose and lactose by ion chromatography with pulsed amperometric detection. Samples are homogenized, buffered and clarified with Carrez reagents after which they are filtered. The resulting solution is diluted and analyzed. The concentration of the various sugars is quantified from the linear regression curve of the standard solutions. All free nutritional sugars stated in the SMPR are included in the method. Selected ingredients and foods were well documented; many potential interferents were tested. Figure 9 gives an overlay of all interferents which shows that there is co-elution of raffinose/stachyose with maltose, ribose with maltitol and xylose/arabinose, galactose with lactitol. The effect of this co-elution is not further discussed, which is important for suitability. Would a different type of column solve the issue? Yes, the analytical technique meets the SMPR, however, it cannot be judged whether all performance characteristics meet the requirements, since the overview of system suitability parameters has not been given.

II. Review of the Method Only 1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used.

Yes partially. The method submission states reproducibility (but as far as I could see it was not analyzed in different laboratories with different operators and different instruments), so all values are assumed being intermediate reproducibility. A summarizing table of all performance characteristics is missing. What is precision, what is specificity, what is stability, LOD/LOQ, etc?