July 2019 Sugar ERP - Review Book

4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s). III. Review of Supporting Information 1. Are the definitions specified in the SMPR used and applied appropriately in the supporting documentation (manuscripts, method studies, etc...)? If not, please explain the differences and if the method is impacted by the difference. 2. Is there information demonstrating that the method meets the SMPR Method Performance Requirements using the Reference Materials stated in the SMPR? If not, then specify what is missing and how this impacts demonstration of performance of the method. method performs within the SMPR Method Performance REquirements table specifications for all analytes in the SMPR applicability statement? If not, please specify what is missing and whether or not the method's applicability should be modified. IV. General Submission Package 1. Based on the supporting information, were there any additional steps in the evaluation of the method that indicated the need for any additional precautionary statements in the method? 3. Is there information demonstrating that the

The safety paragraph is very limiting whereas it only refers to the MSDS of the chemicals (which is/should be good practice in any laboratory). It would be good to address those chemicals that need special attention.

Yes, CRMs are tested and different types of products from the pyramid are included in the study

The method does describe in text the analytical range (0.01-44%), however, there is no clear table that compares all parameters of the method to the SMPR. Would be good to split data in analytical range as given in SMPR and then for each sugar give recovery and RSDr values. It is difficult to judge whether the method meets the SMPR since it lacks this overview.

See previous comment. The data is available, but it needs extra effort to get the information. Request to the authors to extract the numbers from all data and summarize it in a table.

It seems that co-elution occurs between some of the potential interferents (as listed in the SMPR in table 2) and figure 9 in the method. Nothing is stated about effect of these interfering components on quantification of the sugars in a sample.