AOAC ISPAM Meeting eBook, March 17 2015
Salmonella Methods Harmonization Working Group Members
FDA - CFSAN
Thomas Hammack (Chair)
Patrice Arbault
Nexidia
Marcia Armstrong
QIAGEN Gmbh
Mike Clark
Bio-Rad Laboratories Q Laboratories, Inc.
Erin Crowley
Leanne DeWinter Philip Feldsine
Health Canada
BioControl Systems, Inc.
Netherlands Food and Consumer Product Safety Authority
Paul In't Veld Irene Iugovaz
Health Canada
Balamurugan Jagadeesan
Nestec S.A bioMerieux
Ron Johnson Adrianne Klijn
Nestle Quality Assurance Laboratory
W d L
en y auer
Bi R d L b t i o- a a ora or es, nc. I
Wendy McMahon
Silliker Inc.
Sam Mohajer
Canadian Food Inspection Agency
Kirsten Mooijman
Coordinator EURL-Salmonella
Mark Mozola
Neogen Corporation
Brooke Schwartz Meredith Sutzko Morgan Wallace
Brooke Schwartz Consulting
Romer Labs
DuPont Nutrition & Health
Testing for Salmonella
FSIS (USDA) MLG 4.04
FDA BAM Chapter 5
AOACOMA 200.06, 995.20and 967.26
MFHPB 20
ISO 6579
Pre-enrichment in Lactose, Nutrient, UP,
Pre-enrichment in
Pre-enrichment in Lactose or Broth TSB
Pre-enrichment in
Pre-enrichment in BPW or nutrient broth
BPW
or TS broth. Plus others
BPW
Incubation at 35 ºC for 24 hours
Incubation at 35 ºC for 20 to 24 hours
Incubation at 35 ºC for 20 to 24 hours
Incubation at 37 ºC for 16-20 hours.
Incubation at 35 ºC for 18 to 24 hours
Selective enrichment in TT and RV Broth at 35 ºC and 42 ºC and 1 mL and 0.1 mL respectively for 24 h. SC broth at 35˚C for Guar Gum and S. Typhi
Selective enrichment in RV and MKTTn Broth. RVS at 37°C and 42°C and 0.1 mL and 1 mL respectively for 24h
Selective enrichment in TT, RV and / or SC broth depending on method. (1 mL, 0.1 mL and 1 mL respectively) TT and SC are incubated at 35 ºC and RV is incubated at 42 ºC for 24 hours.
Selective enrichment in RVS and TBG broth at 42.5 ºC for 24 h (0.1 mL and 1mL respectively)
Selective enrichment in TTh and mRV, R10, or RVS broth at 42 ºC for 18-24 h (0.5 mL and 0.1 mL respectively)
Streak onto XLD and one other agar (the second agar is any agar for the isolation of salmonella)
Streak onto HE, XLD, BismuthSulfite agar.
BismuthSulfite Agar, HE agar, XLD agar
Streak on at least 2 of the 3: Bismuth Sulfite Agar, BGS, Brilliance Salmonella agar.
Streak onto BGS plus one of DMLIA or XLT 4
Biochemical and serology tests to confirm
Biochemicaland serology tests to confirm
Biochemicaland serology tests to confirm
Biochemical and serology tests to confirm
Biochemicaland serology tests to confirm
Slide Courtesy Donna Douey, Canadian Food Inspection Agency
Made with FlippingBook