Biophysical Society 60th Annual Meeting

1794-Plat 12:15 pm RNA SECONDARY AND TERTIARY STRUCTURE PREDICTION BY TRACING NUCLEOTIDE CO-EVOLUTION WITH DIRECT COUPLING ANALYSIS.  Eleonora De Leonardis, Benjamin Lutz, Sebastian Ratz, Cocco Simona, Remi Monasson, Martin Weigt, Alexander Schug 1795-Plat 12:30 pm MECHANICAL FORCE AND SALT EFFECTS ON THE THERMODYNAMICS OF A FRAMESHIFTING RNA PSEUDOKNOT.  Naoto Hori , Natalia A. Denesyuk, D. Thirumalai

1782-Plat 11:15 am LIGAND-SPECIFIC CONFORMATIONAL CHANGES IN CCR7 COUPLED TO SELECTING DIFFERENT SIGNALING PATHWAYS UPON CCL19 AND CCL21 LIGAND BINDING.  Zied Gaieb , David D. Lo, Dimitrios Morikis 1783-Plat 11:30 am PROTEORHODOPSIN ACTIVATION CAPTURED BY MOLECULAR DYNAMICS SIMULATIONS.  Jun Feng , Blake Mertz 1784-Plat 11:45 am COORDINATED DYNAMICS ORCHESTRATING THE DNA RE-LIGATION BY DE-POISONED TOPOISOMERASE II.  Nan-Lan Huang , Jung-Hsin Lin 1785-Plat 12:00 pm FROM PHYSICS TO PHENOTYPE: NEW INSIGHTS INTO ALLOSTERIC TRANS- PORT MECHANISMS IN LEUT. Michael V. LeVine , Michel A. Cuendet, George Khelashvili, Harel Weinstein 1786-Plat 12:15 pm PLEIOTROPIC ROLE PLAYED BY THE PDZ DOMAIN IN NEURONAL SIGNAL- ING PATHWAYS.  Célia Caillet-Saguy, Pierre Maisonneuve, Florent Delhommel, Henri Buc, Monique Lafon, Muriel Delepierre, Florence Cordier, Nicolas Wolff 1787-Plat 12:30 pm HYDROPHOBIC INTERACTIONS ELICIT COOPERATIVE RESPONSE IN DYSTROPHIN.  Alessandro Cembran , Anne Hinderliter, Benjamin T. Horn, Caitlin T. Pederson, Katie L. Schneider, Jesse A. Skogstad Platform RNA Structure and Dynamics 10:45 am - 12:45 pm, Room 511ABC Co-Chairs Ruben Gonzalez, Columbia University Luis Marky, University of Nebraska Medical Center 1788-Plat 10:45 am INTERROGATION OF CRISPR DYNAMICS WITH FLUORESCENT SINGLE GUIDE RNAS IN LIVE CELLS.  Hanhui Ma, Li-Chun Tu, Ardalan Naseri, Shaojie Zhang, Maximilliaan Huisman, David Grunwald , Thoru Pederson 1789-Plat 11:00 am DECIPHERING THE INFLUENZA A MULTI-SEGMENT GENOME COMPLEX PACKAGING USING SINGLE CELL HIGHLY MULTIPLEXED FISH DATA.  Simon Prisner , Ivan Haralampiev, Matthias Schade, Jasmine Chamiolo, Fabian Jolmes, Oliver Seitz, Andreas Herrmann 1790-Plat 11:15 am EXPLORING THE LIMITS OF A KNOWLEDGE-BASED COARSE-GRAINED MODEL FOR RNA.  Simon Poblete 1791-Plat 11:30 am HIGH THROUGHPUT CHARACTERIZATION OF RNA TERTIARY ELEMENTS.  Sarah Denny , Namita Bisaria, Joseph Yesselman, Rhiju Das, Daniel Herschlag, William Greenleaf 1792-Plat 11:45 am LABEL-FREE, HIGH-TIME-RESOLUTION, SINGLE-MOLECULE STUDIES OF RIBOSWITCH FOLDING.  Nathan S. Daly, Jason J. Hon, Steven B. Warren, Scott M. Trocchia, Colin Nuckolls, Kenneth L. Shepard, Ruben L. Gonzalez Jr 1793-Plat 12:00 pm A COMPARATIVE INVESTIGATION OF THE STABILITY OF DNA AND RNA PSEUDOKNOTS.  Calliste Reiling-Steffensmeier, Luis A. Marky

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Exhibitor Presentation Bruker Nano Surfaces 11:30 am - 1:00 pm, Room 505 BioScope Resolve BioAFM – Unrivalled AFM Biomechanics and Resolution

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In this presentation we will introduce new capabilities for cell mechano- biology and highest resolution cell and molecular imaging available on the BioScope Resolve BioAFM. We will explain how innovations in force control and instrument design have enabled BioScope Resolve to be the first AFM to image microvilli on live cells and to consistently resolve the double helix of DNA, while on the inverted microscope. To enable highest resolution and accurate cell mechanics data, the entire AFMmechanical loop of BioScope Resolve has been designed for stability, specifically on biological samples, with biological sample carriers, and when mounted on the inverted microscope. Designed for Bruker’s exclusive PeakForce Tapping, BioScope Resolve eliminates the need for fluid cantilever tuning entirely with ScanAsyst and provides quantifiable pN force control for imaging and force mapping. The combination of PeakForce Tapping and FASTForce Volume provides the broadest range of frequencies for mechanical characterization, with a new no-touch calibration method guaranteeing accurate calibration. As we will show in the presentation, BioScope Resolve also features synchronization of these unique mechan- ical measurements with fluorescence, enabling new kinds of correlative studies. Speaker Marcin Walkiewicz, Applications Scientist, Bruker – Atomic Force Micros- copy Business Research Programs at PUIs Founding, Establishing, and Maintaining a Research Laboratory 12:00 pm - 1:30 pm, Room 408A This session, sponsored by the Education Committee, provides guid- ance on founding, establishing, and maintaining a research laboratory at Primarily Undergraduate Institutions. Panelists Ashley R. Carter, Amherst College Christine P. Piro, Franklin and Marshall College Alex Small, California State Polytechnic University, Pomona Paul Urayama, Miami University Moderator Scott Brewer, Franklin and Marshall College Postdoc to Faculty Q&A Transitions Forum and Luncheon 12:00 pm - 2:00 pm, Room 510/512 This question-and-answer luncheon, sponsored by the Committee for Professional Opportunities for Women (CPOW), is designed for postdocs finishing and actively applying for academic faculty positions. New faculty and recently tenured faculty in basic science and/or medical school departments will lead the discussion, as well as experienced senior-level faculty who have served as department chairs and/or part of faculty

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