Biophysical Society 60th Annual Meeting

Room 513: Monday, February 29

12:30 pm–2:00 pm Nanion Technologies GmbH Ion Channel Drug Discovery - Beyond the Bottlenecks and Ready for CiPA Nanion Technologies is one of the leading providers of automated patch clamp systems, offering a diverse product portfolio ranging from single channel recordings to HTS-compatible ion channel drug discov- ery. During this workshop, we will show how to push the boundaries of patch clamp-based ion channel high throughput screening projects of various voltage- and ligand gated targets, and how to get ready for CiPA- compliant safety screening going well beyond hERG. Cardiac arrhythmic risk assessment is a hot topic these days calling for new screening strategies. With the CiPA-initiative, the panel of cardiac ion channels to consider have drastically expanded, consequently requir- ing increased data throughput for early compound safety prediction. The SyncroPatch 384/768PE , an automated patch clamp platform recording from up to 768 cells simultaneously, allows the highest data throughput on the market supporting HTS of ion channel active com- pounds and early safety assessment on cardiac channels. Examples will be shown, where six different cardiac channels were recorded using one single plate, in one single run. Patchliner , a medium-throughput APC platform, supports automated current clamp recordings, experiments at physiological temperatures, and a minimal cell usage, making it the ideal partner for safety testing on stem cell derived cardiomyocytes. Additionally, the CardioExcyte 96, a hybrid system combining impedance-based and EFP recordings from beating cardiomyocyte networks from 96 recording wells in parallel, has proven a versatile tool for safety and toxicity screening applications serv- ing as a powerful tool complementing APC. The SURFE2R technology allows direct and functional measurements electrogenic transporter. Hands-on experiments on the SURFE2R will be shown. Also membrane fragments from Chantest, a Charles River com- pany, will be used. Join our workshop to learn more about new safety screening strategies and how to keep up with the increasing demands on cardiac safety and toxicity screening. Speakers

10:30 am–12:00 pm Wyatt Technology Corporation Get it Right the First Time - Enhancing Protein Binding and Structural Studies with the Light-Scattering Toolkit Biophysical binding studies utilizing surface plasmon resonance (SPR), bio- layer interferometry (BLI), isothermal titration calorimetry (ITC), and relat- ed techniques are central to the study of protein-protein, protein-DNA and similar biomolecular interactions. Though these are well-established techniques, in a variety of circumstances, binding measurements may be ambiguous or even fail to provide useful data. Wasted measurements can end up being costly in terms of consumables and time. Small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS) are powerful techniques for studying biomolecular structure. SAXS and SANS usually require precious beam time at large facilities, leaving little room for error where the sample preparation is concerned. Poor samples provide poor SAXS/SANS data, but the opportunity to utilize the X-ray or neutron beam may never be recovered. One thing that SAXS and SANS have in common with SPR, BLI and ITC, is the urgent need to verify sample quality and aggregation state in solu- tion prior to carrying out structural or binding measurements. This semi- nar discusses a suite of complementary techniques, all based on light scattering, that are useful in assessing and troubleshooting many of the underlying characterization issues. Multi-angle light scattering (MALS) and dynamic light scattering (DLS) can help researchers assess solu- tion quality prior to running binding or structural experiments, qualify aggregation behavior of analytes, and characterize complex interactions that may not be amenable to standard characterization methodology. Judicious use of the biophysical light-scattering toolkit is essential for robust and reliable interaction and structure studies. Speaker Sophia Kenrick, Application Specialist, Wyatt Technology Corporation

Maria Barthmes, Nanion Technologies GmbH Andrea Brüggemann, Nanion Technologies GmbH Niels Fertig, Nanion Technologies GmbH Markus Rapedius, Nanion Technologies GmbH

169 16