Biophysical Society 60th Annual Meeting

118-Symp10:45 am MAPPING CONSTRAINTS ON PROTEIN EVOLUTION.  Jesse Bloom 119-Symp11:15 am UNCOVERING AND REPROGRAMMING G PROTEIN COUPLED RECEPTOR SIGNALING.  Patrick Barth 120-Symp11:45 am DESIGNING PEPTIDE INHIBITORS OF ANTI-APOPTOTIC BCL-2 FAMILY PROTEINS.  Amy E. Keating No Abstract 12:15 pm PROTEIN MECHANICS: THE LINK BETWEEN STRUCTURE AND FUNCTION.  Rama Ranganathan Platform HIGH-RESOLUTION STRUCTURAL INSIGHT INTO THE MYOSIN VI-F-ACTIN INTERFACE.  Laura Y. Kim, Pinar S. Gurel, Tosan Omabegho, Zev Bryant, Gregory M. Alushin 122-Plat 11:00 am USING CRYO-EM TO UNTANGLE THE CONFORMATIONAL LANDSCAPE OF A SMALL ALLOSTERICALLY-REGULATED COMPLEX. Mario J. Borgnia , Soojay Banerjee, Alberto Bartesaghi, Doreen Matthies, Prashant Rao, Alan Merk, Jason Pierson, Jacqueline L. Milne, Sriram Subramaniam 123-Plat 11:15 am THE STRUCTURE OF THE METHANOSPIRILLUM HUNGATEI FLAGELLUM AS DETERMINED BY CRYO ELECTRON MICROSCOPY.  Nicole Poweleit 124-Plat 11:30 am IN SILICO REDUCTION OF CONFORMATIONAL VARIANCE IN CRYO-EM IMAGING.  Gunnar F. Schröder , Michaela Spiegel, Amudha Kumari Duraisamy 125-Plat 11:45 am COLLAPSE OF INDIVIDUAL DNA CHAINS CONFINED IN BACTERIOPHAGE CAPSIDS.  Françoise Livolant , Amélie Leforestier, Marta De Frutos, Dominique Durand 126-Plat 12:00 pm STRUCTURAL REMODELING OF BACTERIOPHAGE Φ29 DURING INFEC- TION OF GRAM-POSITIVE BACTERIUM. Madeline M. Farley , Jiagang Tu, Ian Molineux, Jun Liu 127-Plat 12:15 pm CRYO-STEM TOMOGRAPHY PROVIDES MORPHOLOGICAL AND CHEMICAL CHARACTERIZATION OF PRECIPITATED CALCIUM-PHOSPHATE CLUSTERS SEQUESTERED IN MITOCHONDRIA OF INTACT VITRIFIED FIBROBLASTS.  Sharon G. Wolf , Yael Mutsafi, Ben Horowitz, Michael Elbaum, Deborah Fass 128-Plat 12:30 pm ELECTRON CRYO-TOMOGRAPHY OF NANOWIRES IN SHEWANELLA ONEIDENSIS MR-1.  Poorna Subramanian , Sahand Pirbadian, Mohamed Y. El-Naggar, Grant J. Jensen Cryo-Electron Microscopy 10:45 am - 12:45 pm, Room 502A Co-Chairs Gunnar Schroeder, Forschungszentrum Juelich, Germany Sharon Wolf, Weizmann Institute of Science, Israel 121-Plat 10:45 am

resolution in all three dimensions – resulting in a 5x smaller confocal volume. Or push the sensitivity beyond the limits of all conventional confocal microscopes; or use the increase in signal-to-noise ratio to speed up your image acquisition. Traditionally, deeply imaging into intact tissue typically requires multi- photon excitation to penetrate deeper than near the surface of a tissue. Using a “clearing” method to remove the light obstructing opaque molecules from a tissue has been another technique for deep imaging. Techniques such as SCALE, CLARITY, ClearT, SeeDB, CUBIC and others have allowed researchers to image deeper than a millimeter into cleared animal model brains and organs. ZEISS Lightsheet Z.1 features high speed image acquisition and greatly reduced photo damage making imaging of live developmental samples and fixed and cleared tissues easier than ever before. Come learn about using the innovative ZEISS Lightsheet Z.1 microscope for imaging of fixed and cleared tissues. Speakers Joseph Huff, Product Marking Manager, Laser Scanning and Superresolu- tion Microscopy, Carl Zeiss Microscopy LLC Scott Olenych, Product Marketing Manager, Imaging Products, Carl Zeiss Microscopy LLC International Relations Committee Meeting 10:30 am - 12:00 pm, Room 410 Symposium New and Notable 10:45 am - 12:45 pm, Petree Hall C Co-Chairs Vasanthi Jayaraman, University of Texas Health Science Center, and E. Michael Ostap, University of Pennsylvania No Abstract 10:45 am STRUCTURE OF A TRPV2 ION CHANNEL, Seok-Yong Lee No Abstract 11:02 am CRYO-EM STRUCTURE OF A MICRONUTRIENT TRANSPORTER WITH UN- USAUL ARCHITECTURE. Filippo Mancia No Abstract 11:19 am STRUCTURAL DYNAMICS OF K CHANNEL GATING REVEALED BY SINGLE MOLECULE FRET. Colin Nichols No Abstract 11:36 am THE MOTIONS AND INTERACTIONS IN MISMATCH REPAIR TAGET SEARCH DYNAMICS ARE REVEALED BY LIVE-CELL SINGLE-MOLECULE MICROS- COPY. Julie Biteen No Abstract 11:53 am INTEGRATIVE STRUCTURAL BIOLOGY OF TETRAHYMENA TELOMERASE. Juli Feigon No Abstract 12:10 am INTRINSICALLY DISORDERED PROTEINS AS PHYSICAL DRIVERS OF MEM- BRANE TRAFFIC. Jeanne C. Stachowiak No Abstract 12:27 pm FLOPPY BUT NOT SLOPPY: DECODING PLASTICITY IN THE DARK PRO- TEOME OF THE NUCLEAR TRANSPORT MACHINERY. Edward Lemke Symposium Computational and Experimental Approaches to Protein Design 10:45 am - 12:45 pm, Petree Hall D Chair Rama Ranganathan, University of Texas, Southwestern

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