Biophysical Society 60th Annual Meeting

Career Center Workshop Successfully Navigating the International Job Search 4:00 pm - 5:00 pm, Room 518 Applying for a job in one country while finishing up your education and training in another can be challenging, but it can be done with success. In this workshop we will discuss specific strategies to finding jobs in another country while one is abroad and how to leverage your networks in- country to access opportunities, especially those that are hidden. Special emphasis will be placed on establishing your reputation as a leader in your field with professionals in the country or region in which you wish to work. Case studies will be shared. Symposium Molecular Mechanisms of Mechanosensation 4:00 pm - 6:00 pm, Petree Hall C Chair Robert Fettiplace, University of Wisconsin-Madison 975-Symp 4:00 pm GLOBAL AND SPECIFIC INTERACTIONS BETWEEN MECHANOSENSITIVE ION CHANNELS AND THE LIPID BILAYER.  Boris Martinac 976-Symp 4:30 pm SINGLE MOLECULE FORCE SPECTROSCOPY OF HAIR-CELL TIP-LINK PRO- TEINS. Mounir A. Koussa, AndrewWard, Marcos Sotomayor, Wesley P. Wong, David P. Corey 977-Symp 5:00 pm LOCALIZATION OF ANOMALOUS MECHANO-SENSITIVE ION CHANNELS IN COCHLEAR HAIR CELLS. Maryline Beurg, Adam Goldring, Robert Fettiplace No Abstract 5:30 pm STRUCTURE AND CHEMICAL BIOLOGY OF MECHANOSENSITIVE K2P CHANNELS.  Daniel L. Minor Symposium Chair Jane Clarke, University of Cambridge, United Kingdom 978-Symp 4:00 pm ASSESSING AND MANIPULATING PROTEIN FOLDING DYNAMICS.  Feng Gai No Abstract 4:30 pm COUPLED PROTEIN FOLDING AND BINDING REACTIONS: MECHANISMS AND SPEED LIMITS.  Thomas Kiefhaber 979-Symp 5:00 pm IMPACTS OF CHARGE PATTERNING ON INTRINSICALLY DISORDERED PROTEINS AND MECHANISMS OF DISORDER-TO-ORDER TRANSITIONS.  Rohit V. Pappu 980-Symp 5:30 pm THE ROLE OF DISORDER IN PROTEIN FOLDING.  Jane Clarke Folding Rates and Routes 4:00 pm - 6:00 pm, Petree Hall D

Exhibitor Presentation Renishaw Inc 2:30 pm - 4:00 pm, Room 513

M O N D A Y

Innovative Raman Imaging in the Life Sciences When light illuminates a sample, most of it scatters without changing. A tiny fraction of the light however is Raman scattered. The Raman scat- tered light excites the phonons in the samples and produces a spectrum. This spectrum tells us how the atoms are vibrating, providing a chemical fingerprint which allows identification of the sample. Raman spectros- copy produces chemical and structural information to help us under- stand more about the material being analyzed. The ability to probe the chemical and molecular structure of biological materials is obtained directly without the need for any dyes or markers. These systems can be utilized to generate chemical images of cells, tissue, bone and bio-com- patible materials with very high spatial resolution. It has been employed for cancer diagnosis, stem cell differentiation, skin treatments, protein structure analysis, bio-diagnostics and bacterial identification. Renishaw’s inVia confocal Raman microscope can be integrated with other instruments, such as atomic force microscopy (AFM) and scanning electron microscopy (SEM), to provide Raman analysis from the same point on the sample. This talk will provide an introduction to Raman mi- croscopy with biological materials, the instrumentation required for these techniques and will highlight some applications where Raman microscopy is making the biggest impact with biological materials . Speakers Tim Prusnick, USA Sales Manager SPD, Renishaw Inc Andrew King, Regional Sales Manager - West Coast, Renishaw Inc Mark Canales, Field Applications Specialist (Life Science) Spectroscopy Products Division, Renishaw Inc Membership Committee Meeting 3:00 pm - 5:00 pm, Room 506 Exhibitor Presentation Bruker Nano Surfaces 3:30 pm - 5:00 pm, Room 505 Advances in Live Super-Resolution Imaging Using the Vutara 352 Micro- scope Super-resolution microscopy has made a significant impact in the field of biological imaging by enabling a ten-fold improvement in spatial reso- lution over traditional light microscopy techniques. Most of the imaging has been so far targeted at fixed specimens with a few live cell applica- tions. The Vutara 352 microscope has been engineered towards live-cell imaging by enhancing spatial and temporal resolution in single molecule localization super-resolution. The sCMOS detector in the Vutara 352 enables imaging at 800 fps at full ROI and at video frame rates at reduced ROI. Two color simultaneous imaging can be applied in both super-reso- lution live cell and 3D particle tracking experiments. The biplane based detection path enables imaging thicker samples such as whole mount Drosophila and offers deeper penetration into tissues. The Vutara 352 also includes real time localization along with several statistical and live cell analysis features for processing data. In summary, the Vutara 352 microscope is a powerful super-resolution imaging and analysis tool. Speaker Manasa Gudheti, Applications Scientist at Bruker – Fluorescence Micro- copy Business

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