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8.8 Soil Storage 457 physical, chemical, microbiological, and biochemical (protein and nucleic acid) 458 characteristics and composition. Ideally, subsamples should be archived with associated 459 collection, processing, and characterization metadata in a searchable database. 463 in a closed-lid container for up to five years. Before using the soil for experiments, the 464 soil should be well mixed. Soils should also be re-characterized prior to experiments 465 every 2 years. 469 immediately after collection or as soon as possible after that time. Until usage, the 470 samples should be stored at 4°C in a sealed container to limit microbial activity without 471 having a major impact on the microbial community composition. Studies show that 472 storage for more than 21 days at 4°C begins to impact some of the measurable microbial 473 activities (10). Once processed, soil samples should be subdivided and stored to preserve the 8.8.1 Air-Dried Soils Soils that are processed by air drying and sieving can be stored at room temperature 8.8.2 Soils for Microbiological and Biochemical Applications Soils that are used specifically for their microbial community should be used either

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8.9 Manufactured Soil

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