SPADA Meeting Book

Background

• Microbial cultures are dynamic systems, which can undergo  significant changes as they are handled and propagated in  laboratory settings. • While storage, propagation (passing), and distribution is  necessary to ensure the availability of the organism for  basic and applied research, these processes can make it  difficult and misleading to compare experimental results  obtained from different locations and times. • The careful handling and verification of microbial cultures is  essential for comparing experimental results.

Document Key Points

• Definitions

• Roles and 

Responsibilities – Study sponsor – Culture producer – Performer • Culture Verification  Statement

– Extensible study – Test and index  cultures

– Culture verification – Propagation history – Orthogonal tests – Application oriented  tests

– Propagation history – Orthogonal testing – Application oriented  testing