SPADA Meeting Book

In-silico Document Key Points

• Rationale of why modern approaches are needed • Comparison of Traditional vs Modern assay  development Process • Assay Design – Traditional – Modern • Creation of relevant databases • In silico tools for design • Metrology and assay stewardship • Wet lab testing and Validation of Assays • Regulatory approval guidelines

Comments Submitted

• DG°T, DH°, DS° ‐ These probably need to be defined  (section 4.3.5‐ line 383). • "different scoring methods" ‐ So the goal isn’t to  determine which models worked best but rather  how to put a score on a model?  That isn’t what it  sounded like at the beginning of this section.   Perhaps the goal should be more clear at the onset  (section 5.3‐ line 629). • "58 of 60 test replicates" ‐ These numbers probably  need additional information to back them up.  I  suggest not providing specific numbers in this  example.  It might be misleading (section 6.1‐line  660).