SPADA Meeting Book
191 Figure 4. Traditional vs. modern assay development pipeline. Inc/Exc: Inclusivity/Exclusivity.
192 193
4.0 Assay Design
194
195
4.1 Target selection: user defined vs unbiased (in silico)
Traditionally, assay target selection has been an ill-defined process that is strongly influenced 196 by the preferences and experience of individual assay designers. Often, assay targets are selected 197 from lab-specific research interests on specific genes of given pathogens or from known 198 (literature-based), or suspected, virulence factor genes. The resulting assays are then screened 199 (either computationally or “by eye”) for inclusivity and exclusivity using hand-selected 200 sequences. The resulting assays are also often broadly screened computationally against all 201 known sequences (e.g., by using Basic Local Alignment Search Tool ( BLAST ) to identify 202
12
Made with FlippingBook - Online magazine maker