Press Kit

Raman Chemometric Urinalysis (Rametrix TM ) Screen for Chronic Kidney Disease

Medical studies over the past 40 years have shown that many forms of kidney disease are progressive, potentially leading to end-stage kidney disease and renal failure. These studies have also shown that early detection of chronic kidney disease (CKD) and use of medical and lifestyle interventions may slow the progression of CKD and reduce associated healthcare costs, which approach $98B annually (about 15% of the Medicare budget). DialySensors, Inc. has recently invented and extensively validated Raman Chemometric Urinalysis (Rametrix™) to analyze urine and to screen for the presence and stage of CKD. The Rametrix TM screen has >95% sensitivity and >95% specificity for detecting late-stage CKD. Urine is a complex biological fluid containing thousands of molecules that reflect the body’s physiologic/metabolic state. CKD interferes with waste elimination. Thus, the composition of urine for every individual accurately reflects renal health and can provide a means for early detection and accurate staging of CKD. Rametrix ™ detects changes in urine molecular composition, which is associated with nephron deterioration (i.e., CKD). Rametrix™ technology uses off -the-shelf, low-profile Raman spectrometers (~$20K per instrument), our proprietary computational tools, and our extensive patient database. No sample preparation of urine specimens is required, only a single collection is needed, results are available in about 5 minutes, and the analysis can be performed for less than $1 per specimen.

Urine Specimen

Raman Spectroscopy

CKD Status and Stage are Unknown

Strong Urea Band

5 minutes

Glass vial (no sample preparation)

New Raw Raman Spectrum

Collection cup

Agiltron ® PeakSeeker TM ProShown http://www.ramansystems.com/english/peakseeker.htm

Combine with Spectra in Rametrix TM Database

Rametrix TM Clustering

Rametrix TM Computational Pipeline

Healthy Individuals (Blue Data Points)

Urine Specimen After Rametrix TM Analysis

Packaged as MATLAB ® Toolbox (Expanding to R and Python TM )

CKD Patients (Red Data Points)

Fluorescence Removal; Multivariate Statistics; Cluster Analysis

Spectra in Rametrix TM Database

CKD Stages are Known

Quantification and Report Generation

Rametrix TM Screen Results

CKD Stages Relative to Healthy

Stage 5

Healthy

Stage 2 Stage 1

Stage 4 Stage 3

100 90 80 70 60 50 40 30 20 KRam Score (0-100 Rams scale)

0 10

Example Result: KRam Score = 21.5 Rams /100 (CKD Stage 1)

© 2018, DialySensors, Inc.

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