AOAC ERP MICRO AUGUST 2018

OMAMAN-44 A: Collaborative Study Manuscript Expert Review Panel Use Only August 2018

Pre-collaborative and Collaborative Report Peel Plate EB

Table 3: Summary of Categories, Types, Items, Strains and Inoculation Levels for the Matrix Study

Inoculating Organism (Culture Conditions)

Replicates/ Test Portion Size

Food Category

Food Type

Food Item

Achieved Contamination Levels a

Enterobacter Amnigenus ATCC b 51816 (Heat Stressed)

10-100 CFU/ml

5 x 25g 5 x 25g 5 x 25g 5 x 25g 5 x 25g 5 x 25g 5 x 25g 5 x 25g 5 x 25g 5 x 25g 5 x 25g 5 x 25g

Pasteurized Milk Based Products

3.25% Pasteurized Whole Milk

Heat Processed Milk and Dairy Products

100-5,000 CFU/ml

5,000-100,000 CFU/ml

10-100 CFU/ml

Hafnia alvei ATCC 51815 (Lyophilized)

Dry Milk Powder

Milk Powder

100-5,000 CFU/ml

5,000-100,000 CFU/ml

10-100 CFU/ml

Infant Formula (milk based) with Probiotics Infant Cereal with Probiotics Stainless steel food contact surface

Cronobacter sakazakii CCUG c 28863 (Lyophilized)

Infant Formula with Probiotics

100-5,000 CFU/ml

Infant Formula and Infant Cereals

5,000-100,000 CFU/ml

10-100 CFU/ml

Escherichia coli ATCC 25922 (Lyophilized)

Infant Cereal with Probiotics

100-5,000 CFU/ml

5,000-100,000 CFU/ml

10-100 CFU/ml

4in x4 in square 4in x4 in square 4in x4 in square

Salmonella Typhimurium ATCC 14028

Environmental Surfaces

NA

100-5,000 CFU/ml

5,000-100,000 CFU/ml

10-100 CFU/ml

Carcass Carcass Carcass

In Process Sample

Carcass Rinse

Chicken

Natural Contamination

100-5,000 CFU/ml

5,000-100,000 CFU/ml a The uninoculated and the low contamination levels were blind-coded and evaluated by ISO 21528-1:2017 reference method. The medium and high contamination levels were blind-coded and evaluated by the ISO 21528-2:2017 reference method. b ATCC-American Type Culture Collection. c CCUG-University of Goteborg Culture Collection. ERP Use Only

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