AOAC ERP MICRO AUGUST 2018

OMAMAN-44 A: Collaborative Study Manuscript Expert Review Panel Use Only August 2018

Pre-collaborative and Collaborative Report Peel Plate EB

Table 7. Peel Plate-EB Method (Duplicate count) for Enterobacteriaceae at 48 hours Compared to ISO Methods 21528-1 and 2.

Fortified micro- organisms ATCC No. Enterobacter Amnigenus ATCC 1 51816 (Heat Stressed)

Candidate Method

Reference Method

95% CI e

Cont. level

Mean diff. d

r 2 h

Matrix

Mean a

s r

RSD r

Mean a

s r

RSD r

LCL f

UCL g

b

c

b

c

None

<0.1

na

na 20

<0.1

na

na 28

na

na

na

3.25% past. whole milk j

1.017 3.429 4.199 1.896 3.504 4.892 1.669 3.612 4.835 2.216 3.203 4.889 <0.1 <0.1 <0.1

Low

1.025 3.489 4.190 1.892 3.604 5.004 1.789 3.642 4.902 2.207 3.181 4.975 1.767 3.246 4.656 1.200 2.503 3.592 <0.1 <0.1 <0.1 <0.1

0.200 0.146 0.107 0.137 0.064 0.159 0.111 0.064 0.087 0.108 0.102 0.116 0.061 0.048 0.024 0.036 0.027 0.028 na na na na

0.289 0.087 0.144 0.204 0.106 0.081 0.232 0.064 0.068 0.146 0.102 0.150 0.136 0.045 0.038 0.099 0.043 0.042 na na na na

0.008 0.059 -0.009 -0.004 0.100 0.113 0.120 0.030 0.067 -0.009 -0.022 0.086 0.059 -0.029 0.018 0.036 0.029 0.021 na na na na

-0.138 -0.106 -0.117 -0.102 -0.008 -0.128 -0.119 -0.001 -0.050 -0.078 -0.131 -0.085 -0.138 -0.071 -0.022 -0.049 -0.013 -0.012 na na na na

0.154 0.224 0.100 0.094 0.207 0.353 na

0.994

Medium

4 3

3 3

High None

na 11

na 11

Non-fat dry milk powder j Infant Formula with probiotic j Infant Cereal with Probiotic j Sponge sample from Stainless Steel j Chicken Rinse in n-BPW j

Hafnia alvei ATCC 51815 (Lyophilized) Cronobacter sakazakii CCUG 2 28863 (Lyophilized)

Low

0.991

Medium

3 2

3 2

High None

na

na 14

na

Low

6 2 2 5 3 2 3 1 1 3 1 1

0.3586

0.992

Medium

2 1

0.062 0.184

High None

na

na

na

Escherichia coli ATCC 25922 (Lyophilized)

Low

7 3 3 7 1 1 9 2 1

0.059 0.088 0.258 0.255 0.013 0.059 0.121 0.072 0.055 na

0.993

Medium

High None

na

<0.1

na

Salmonella Typhimurium ATCC 14028

1.709 3.245

Low

0.997

Medium

High Low ERP Use Only 4.637 1.164 2.474 3.587 Medium High

0.998

Natural

40