AOAC ERP MICRO AUGUST 2018
OMAMAN-44 A: Collaborative Study Manuscript Expert Review Panel Use Only August 2018
Pre-collaborative and Collaborative Report Peel Plate EB
Table 11. Evaluation of Peel Plate EB assay perturbations
Bacterial Strain
Paired T- Test Probability of equivalence
High and Low Condition
Mean cfu/mL
Log Difference a
Assay Perturbation
SD
CV%
LCL b
UCL c
Serratia marcescens ATCC 13880 Citrobacter fruendii ATCC 8090 Serratia marcescens ATCC 13880 Citrobacter fruendii ATCC 8090 Hafnia alvei ATCC 51815 Enterobacter aerogenes ATCC 13048
41 36 31 32 44 60 48 65 21 22
5 7 5 8 7 9
13% 17% 21% 19% 15% 14% 20% 15% 14% 14% 10%
35°C 39°C 35°C 39°C
20%
-0.059
-0.130
0.012
Temperature
54%
0.046
-0.030
0.123
900µL
<0.1%
0.131
0.093
0.170
1100 µL
Pipet Volume
10
900µL
<0.1%
0.135
0.064
0.206
1100 µL 22 hours 50 hours 22 hours 50 hours
9 3 3
6%
0.014
0.007
0.022
Assay Time
100 101
10
16%
0.002
-0.006
0.014
9
9%
a Mean log cfu/mL difference between the low and high pairs n=10 pairs. b 95% Lower confidence limit for difference of means. c 95% Upper confidence limit for difference of means.
ERP Use Only
48
Made with FlippingBook Learn more on our blog