AOAC ERP MICRO AUGUST 2018

OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016

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For each method, the probability of detection (POD) was calculated as the number of positive outcomes divided by the total number of trials. [9] The following were calculated : for the

candidate presumptive results, POD CP

; the candidate confirmatory results, POD CC

; the difference

in the candidate presumptive and confirmatory results, dPOD CP ; results that confirmed positive, POD C ; confirmed candidate and reference methods, dPOD C the reference method, POD R

the presumptive candidate , and the difference in the

. POD analyses were conducted for the

MDA 2 - Salmonella test points and compared to the appropriate reference method results. The pre-validation target analyte background screen results and APC results are presented in Table 5. The heat stress data for selected matrixes are presented in Table 6. The inoculum levels for each environmental surface are presented in Table 7. A summary of POD analyses [10] is presented in Tables 8-23. As required in Appendix J of the Official Methods of Analysis Manual [11],

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fractional positive results were obtained for all matrixes.

Raw Ground Beef (73% Lean) (25 g) – 10 hour Primary Enrichment

For the low inoculation level of the MDA 2 Salmonella method, there were 11 presumptive positives and 11 confirmed positives following the USDA/FSIS MLG 4.08 reference method confirmation procedure. There were 9 observed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 4.08 reference method confirmation procedure. There were 5 confirmed positives following the reference method. Detailed results of the POD analyses are presented in

Tables 8 and 16 of the Appendix.

Raw Ground Beef (73% Lean) (325 g) – 10 hour Primary Enrichment

For the low inoculation level of the MDA 2 Salmonella method, there were 7 presumptive positives and 7 confirmed positives following the USDA/FSIS MLG 4.08 reference method confirmation procedure. There were 9 observed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 4.08 reference method confirmation procedure. There were 5 confirmed positives following the reference method. Detailed results of the POD analyses are presented in

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Tables 8 and 16 of the Appendix.

Raw Ground Beef (73% Lean) (25 g) – 24 hour Primary Enrichment

For the low inoculation level of the MDA 2 Salmonella method, there were 11 presumptive positives and 11 confirmed positives following the USDA/FSIS MLG 4.08 reference method confirmation procedure. There were 9 observed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 4.08 reference method confirmation procedure. There were 5 confirmed positives following the reference method. Detailed results of the POD analyses are presented in

Tables 8 and 16 of the Appendix.

Raw Ground Beef (73% Lean) (325 g) – 24 hour Primary Enrichment