AOAC ERP MICRO AUGUST 2018

OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016

Table 1. 3M MDA 2 – Salmonella Enrichment Protocols Sample Matrix Sample Size Enrichment Broth Volume

Enrichment Temperature (±1°C)

Enrichment Time (hr)

225 mL BPW ISO (pre-warmed) 975 mL BPW ISO (pre-warmed) 2,925 mL BPW ISO 225 mL BPW ISO 1,500 mL BPW ISO

25 g

Raw ground beef, raw ground chicken

41.5

10-24

325 g 325 g

Cooked breaded chicken

37

18-24

25 g

Dry dog food

37

18-24

375 g

Black Pepper, Raw whole shrimp, Raw bagged spinach, Pasteurized processed American cheese Chicken carcass rinse Chicken carcass sponge Instant non-fat dry milk

25 g

225 mL BPW ISO 37

18-24

30 mL BPW ISO (pre-warmed) 50 mL BPW ISO (pre-warmed)

30 mL

41.5

18-24

1 sponge

41.5

18-24

25g

225 mL BPW ISO 37

20-24

Cocoa powder

25g

225 mL BPW ISO 37

24-28

Pasteurized liquid whole egg

100 mL

900 mL BPW ISO 37

18-24

Spent sprout irrigation water

3,375 mL BPW ISO 225 mL BPW ISO 3,375 mL BPW ISO 225 mL BPW ISO (pre-warmed) 10 mL BPW ISO (pre-warmed) 50 mL BPW ISO (pre-warmed)

375 mL

37

18-24

25 g

Creamy peanut butter

37

18-24

375 g

1 sponge AOAC Research Institute Expert Review Panel Use Only 41.5 18-24 1 swab 41.5 18-24 1 sponge 41.5 18-24

Sealed concrete

Stainless steel Sealed ceramic tile

Environmental a Transfer 20 µL into LS tubes for the above table.