AOAC ERP MICRO AUGUST 2018
OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016
Table 4: Matrixes and Inoculating Organisms
MDA 2 Salmonella Testing Time Points
Inoculating Organism (Culture Condition) Salmonella Agona ATCC 1 51957
Matrix/Test Portion Size
# of Replicates
Reference Method (Enrichment)
Target Inoculum Level
0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion
5
Raw Ground Beef (73 % Lean) (25 g & 375 g) Raw Ground Chicken (25 g & 375 g) Chicken Carcass Rinse Chicken Carcass Sponge Instant Non-fat Dry Milk (25 g)
USDA/FSIS MLG 4.08 (mTSB) USDA/FSIS MLG 4.08 (BPW) USDA/FSIS MLG 4.08 (BPW) USDA/FSIS MLG 4.08 (BPW)
10 and 24 hours 14 and 24 hours
20
5 5
Salmonella Heidelberg NCTC 2 5717 Naturally Contaminated 3 Naturally Contaminated 3
20
5
Lot 1 & Lot 2 Lot 1 & Lot 2
20 & 20 20 & 20
18 hours
18 hours
0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion
5
FDA/BAM Chapter 5 (Brilliant Green Water) FDA/BAM Chapter 5 (TSB) FDA/BAM Chapter 5 (Reconstituted non- fat dry milk)
Salmonella Infantis ATCC 1 51741 (lyophilized)
20 hours
20
5 5
Salmonella Tallahassee ATCC 1 12002 (lyophilized)
Black Pepper (25 g)
20
18 hours
5 5 5 5
Salmonella Newport ATCC 1 6962 (lyophilized)
Cocoa Powder (25 g)
24 hours
20
Salmonella Enteriditis ATCC 1 13076 (heat-stressed)
Pasteurized Liquid Whole Egg (100 g) Raw Whole Shrimp (25 g)
USDA/FSIS MLG 4.08 (BPW)
20
18 hours
5 5
Salmonella Virchow ATCC 1 51955
FDA/BAM Chapter 5 (Lactose)
20
18 hours
5 5
Raw Bagged Spinach (25 g)
Salmonella Schwarzengrund STs 4 95
FDA/BAM Chapter 5 (Lactose)
20
18 hours
5 5 AOAC Research Institute Expert Review Panel Use Only Salmonella Saintpaul ATCC 1 9712 Salmonella Kentucky ATCC 1 9263 (heat-stressed) 18 hours FDA Guidance Document (BPW+n) 20 5 5 18 hours USDA/FSIS MLG 4.08 (BPW) 20 5
Spent Sprout Irrigation Water (375 mL) Cooked Breaded Chicken (325 g)
1 ATCC - American Type Culture Collection 2 NCTC-National Culture Type Collection 3 Natural contamination was present during the background screen: 2 separate lots evaluated (20 replicates each) 4 STs- Pennsylvania State University Culture Collection
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