AOAC ERP MICRO AUGUST 2018

OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016

Table 9: 3M ™ MDA 2 - Salmonella Assay, Candidate vs. Reference – POD Results

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

Matrix/Test Portion

N b

Strain

dPODCf 95% CIg

x c

d 95% CI

e

POD C

X

POD R

95% CI

-

5

0 9 5 0 9 5 0 8 5 0 8 5

0.00 0.45 1.00 0.00 0.45 1.00 0.00 0.40 1.00 0.00 0.40 1.00

0.00, 0.43 0.26, 0.66 0.57, 1.00 0.00, 0.43 0.26, 0.66 0.57, 1.00 0.00, 0.43 0.22, 0.61 0.57, 1.00 0.00, 0.43 0.22, 0.61 0.57, 1.00

0 9 5 0 9 5 0 9 5 0 9 5

0.00 0.45 1.00 0.00 0.45 1.00 0.00 0.45 1.00 0.00 0.45 1.00

0.00, 0.43 0.00 0.26, 0.66 0.00 0.57, 1.00 0.00 0.00, 0.43 0.00 0.26, 0.66 0.00 0.57, 1.00 0.00

-0.43, 0.43 -0.28, 0.28 -0.43, 0.43 -0.43, 0.43 -0.28, 0.28 -0.43, 0.43 -0.43, 0.43 -0.33, 0.24 -0.43, 0.43 -0.43, 0.43 -0.33, 0.24 -0.43, 0.43

10 Hours

0.56 (0.29, 0.94) 20

4.38 (1.72, 11.15)

Raw Ground Chicken (25 g)

5

Salmonella Heidelberg NCTC 5717

-

5

24 Hours

0.56 (0.29, 0.94) 20

4.38 (1.72, 11.15)

5

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD C

x c

d 95% CI

e

POD C

X

POD R

95% CI

-

5

0.00, 0.43 0.00 0.26, 0.66 -0.05

14 Hours

0.56 (0.29, 0.94) 20

4.38 (1.72, 11.15)

Raw Ground Chicken (325 g)

5

0.57, 1.00 0.00

Salmonella Heidelberg NCTC 5717

-

5

0.00, 0.43 0.00 0.26, 0.66 -0.05 0.57, 1.00 0.00

24 Hours

0.56 (0.29, 0.94) 20

4.38 (1.72, 11.15)

5

a MPN = Most Probable Number is calculated using the LCF MPN calculator provided by AOAC RI, with 95% confidence interval b N = Number of test portions c x = Number of positive test portions d POD C = Candidate method confirmed positive outcomes divided by the total number of trials e POD R = Reference method confirmed positive outcomes divided by the total number of trials f dPOD C = Difference between the confirmed candidate method result and reference method confirmed result POD values g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level AOAC Research Institute

Expert Review Panel Use Only